Characterization of Extra Cellular Enzymes from Soil Actinomycetes: A Molecular Approach
||International Journal of Biotech Trends and Technology (IJBTT)|
|© 2015 by IJBTT Journal|
|Volume - 5 Issue - 1
|Year of Publication : 2015|
|Authors : Payal Das, Renu Solanki and Monisha Khanna|
Payal Das, Renu Solanki and Monisha Khanna "Characterization of Extra Cellular Enzymes from Soil Actinomycetes: A Molecular Approach", International Journal of Biotech Trends and Technology (IJBTT), V5(1):20-32 Jan - Mar 2015, Published by Seventh Sense Research Group.
Extracellular enzymes from microbes are important biocatalysts with their widespread applications in several industries such as textile, biorefineries, food, pulp and paper, agriculture, detergent and pharmaceuticals. Actinomycetes are group of gram positive soil microbes, well known as producers of extracellular enzymes. Among the various genera of actinomycetes, Streptomyces has been a widely exploited group for production of these enzymes. Primary and secondary screening of isolates for identifying the highest enzyme producers followed by estimation of enzyme activity is the primary step. Immense work has been done for identification of genes, understanding the role of structural domains responsible for enzyme activity by X-ray crystallography, mass spectroscopy and NMR studies. This is followed by analyses of protein sequences, phylogenetic tree construction based on amino acid differences among species and recombinant studies for identification of protein families for hyper-producing the enzyme yield.
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Extracellular enzymes from actinomycetes, Primary and Secondary screening, Structural analysis by Xray crystallography and Mass spectroscopy, Gene and protein phylogeny, Recombinant studies.