Comparative Studies of Callus Cell and Shoot Proliferation From Pineapple And Banana Culture in Vitro: Antioxidant, Carbohydrate, Pigment and Mineal Properties

 
 
International Journal of Biotech Trends and Technology (IJBTT)
 
© 2018 by IJBTT Journal
Volume - 8 Issue - 2                          
Year of Publication : 2018
Authors : ABM Sharif Hossain
  10.14445/22490183/IJBTT-V8I2P601

ABM Sharif Hossain "Comparative Studies of Callus Cell and Shoot Proliferation From Pineapple And Banana Culture in Vitro: Antioxidant, Carbohydrate, Pigment and Mineal Properties", International Journal of Biotech Trends and Technology (IJBTT), V8(2): 1-6 April - June 2018, Published by Seventh Sense Research Group.

Abstract

The study was conducted to evaluate the comparison of the callus cell, shoot and stem proliferation from pineapple crown slice and banana inflorescence in vitro using NAA and BAP at different concentration. The pineapple shoot number and weight was higher in BAP at 2.0 mg/l than control and NAA 0.2 mg/l. However, the BAP + IAA (10mg/l + 1 mg/l) concentration produced higher callus cell weight, shoot number and stem length than MS medium (without hormone) and NAA 1mg/l concentrations in banana. Moreover, it has been shown that the sugar and chlorophyll content were higher in BAP treated hormone than MS medium and NAA 0.2mg/l concentration in pineapple. In addition to that the nutrient content (K+ and NO3--) was exhibited higher in the 2 mg/l BAP treated explants than MS (control) and 0.2 mg/l NAA treated explants in pineapple. Callus tissue weight was maximal in BAP (10mg/l) + IAA (1mg.l) treated explants compared to the NAA (1 mg/l) and MS treated explants in banana. Besides, shoot number and stem length were higher in the BAP (10mg/l) + IAA (1mg/l) treated explants than NAA (1 mg/l) and MS (control) media in banana. The highest sugar and chlorophyll content was recorded in BAP (10mg/l) + IAA (1mg/l) treated explants in banana. Meanwhile, the total phenol content was significantly increased by BAP treatment. K+ and NO3-- content in explants were significantly increased by BAP and NAA treatments in banana. The result showed that the DPPH radical scavenging activity increased with hormone application. Finally it seemed that BAP was the best effective hormone for the both pineapple and banana explants regeneration. Therefore the highest shoot and stem was regenerated in BAP (2mg/l) in pineapple.

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Keywords
callus cell and tissue, proliferation, pineapple crown and banana bud, antioxidant