Use of RAPD and ISSR Markers for Molecular Genetic Analysis of Eucalyptus tereticornis

 
 
International Journal of Biotech Trends and Technology (IJBTT)
 
© 2014 by IJBTT Journal
Volume - 4 Issue - 3 
Year of Publication : 2014
Authors : Devendra Nishad , Priya Singh , Sujeet K. Singh

Devendra Nishad , Priya Singh , Sujeet K. Singh. "Use of RAPD and ISSR Markers for Molecular Genetic Analysis of Eucalyptus tereticornis", International Journal of Biotech Trends and Technology (IJBTT), V4(3):31-33 Jul - Sep 2014, Published by Seventh Sense Research Group.

Abstract

Random Amplified Polymorphic DNA (RAPD) and Inter Simple Sequence Repeat (ISSR) polymorphism was employed to assess the genetic variations in the germplasm of Eucalyptus tereticornis. 15 trees under cultivation were analysed with 10 RAPD primers and 4 ISSR Primers of which all RAPD Primers and only 2 ISSR primers showed reproducible results. Marker attributes like Polymorphism Information Content (PIC), Effective Multiplex Ratio (EMR), and Marker Index (MI) and Informative bands (Ib) values were calculated to assess the discriminatory power of RAPD and ISSR primers. For RAPD primers the PIC values ranged from 0.34 to 0.50, the EMR ranged from 34 to 201, the MI value ranged from 17 to 88.44 with an average of 9.119 per primers. For ISSR primers the PIC values ranged from 0.36 to 0.41 with an average of 0.38 per primer. The EMR ranged from 122 to 129.6 and the MI values ranged from 50.02 to 46.65 with an average of 46.65 per primer. The Ib value ranged from 50 to 56 with an average of 53 per primer combination. The UPGMA-phenogram categorized the 15 trees into two major clusters based on genetic similarity and dissimilarity.

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Keywords
RAPD marker, UPGMA clustering, Polymerase Chain Reaction, EMR, PIC, MI and ISSR Markers.