In Vitro Detection of Uropathogenic Bacteria from UTI Infected Patients and Their Suitable Drugs Selection

 
 
International Journal of Biotech Trends and Technology (IJBTT)
 
© 2014 by IJBTT Journal
Volume - 4 Issue - 3 
Year of Publication : 2014
Authors : Raihanul Islam , Suvamoy Datta , Dayanidhi Sarkar

Raihanul Islam , Suvamoy Datta , Dayanidhi Sarkar. "In Vitro Detection of Uropathogenic Bacteria from UTI Infected Patients and Their Suitable Drugs Selection", International Journal of Biotech Trends and Technology (IJBTT), V4(3):11-27 Jul - Sep 2014. Published by Seventh Sense Research Group.

Abstract

Urinary tract infections (UTI) are one of the most common infectious diseases diagnosed and management of urinary tract infections is complicated by the increasing prevalence of virulent antibiotic-resistant strains. The present study was designed to isolate the virulent organisms and their drug sensitivity status in a certain community. Total 100 urine samples were collected from patients with clinically diagnosed UTI attending both out-door and in-door patient from the prescription point. The isolates were identified by standard microbiological and biochemical process. From the total collected urine samples of the patients, 80% samples showed culture positive. Moreover, female patients were found more infected than male. Escherichia coli, Klebsiella spp & Staphylococcus spp were prevalent in all urine samples isolated from the diagnostic centers and subjected to characterizations namely cultural and biochemical identification, microscopic observation, identification of ESBL positive strain, antibiogram, and hemolytic assay study for the detection of virulence property. In this study 86% E. coli and 40% Klebsiella spp shown positive result for the presence of  lactamase. E. coli, Klebsiella spp & Staphylococcus spp. showed multiple resistances of 6 to 8 antibiotics. E. coli and Klebsiella spp were 100% sensitive to imipenem and amikacin and compared to the other antibiotics tested and therefore these may be the drugs of choice for the treatment of UTIs.

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Keywords
Urinary tract infections, drug resistance, Escherichia coli, Extended spectrum -lactamases (ESBL).