Evaluation of In-vitro Antioxidant and Xanthine oxidase inhibitory activity of selected Indian plants

 
 
International Journal of Biotech Trends and Technology (IJBTT)
 
© 2012 by IJBTT Journal
Volume - 2 Issue - 4                          
Year of Publication : 2012
Authors :Rajeswari Hari, Vasuki. R, Gyan Prakash Prasoon, Hari Prakash Singh, Nishant Mishra

Rajeswari Hari, Vasuki. R, Gyan Prakash Prasoon, Hari Prakash Singh, Nishant Mishra "Evaluation of In-vitro Antioxidant and Xanthine oxidase inhibitory activity of selected Indian plants",International Journal of Biotech Trends and Technology (IJBTT), V2(4):1-9 October - December 2012. Published by Seventh Sense Research Group.

Abstract

In the present investigation an attempt was made to find a new herbal derived material with potential Xanthine oxidase inhibitory and free radical scavenging activities. We have screened the Ethanolic extracts of 10 medicinal plants belonging 8 families, regardless of their claimed ethanopharmacological and /or food uses for their XO inhibitory activities using an optimized protocol. The Super oxide, Nitric oxide, hydrogen peroxide radical scavenging activity and Total antioxidant activity of these extracts were investigated employing various established in vitro systems. Total phenolic and flavanoid content were also determined. The Xanthine oxidase enzyme inhibitory and the antioxidant activity of Ethanolic plant extract were found to be in the following order. Piper nigrum > Brassica juncea > Cuminum cyminum > Cinnamomum zeylanicum > Coriandrum sativum > Cinnamomum tamala > Nigella sativa > Elettaria cardamomum > Syzygium aromaticum > Prunus amygdalus. Among these plant extracts the three plants namely Piper nigrum ,Cuminum cyminum and Brassica juncea were found to be most active for the above said activities. The quantitative estimation of revealed the considerable amount of phenols and flavanoids which may be attributed for its antioxidant activity through the inhibition of Xanthine oxidase enzyme. The study showed that many of the tested plant species are potential sources of natural XO inhibitors that can be developed, upon further investigation, into successful herbal drugs for treatment of gout and other XO-related disorders.

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Keywords
Free radical scavenging, Total antioxidant, Xanthine oxidase.