Successful Propagation of Pigeonpea (Cajanus cajan L.) through In Vitro Shoot Regeneration and Ex Vitro Rooting

 
 
International Journal of Biotech Trends and Technology (IJBTT)
 
© 2017 by IJBTT Journal
Volume - 7 Issue - 3                          
Year of Publication : 2017
Authors : Atul Dev, Ajinder Kaur
  10.14445/22490183/IJBTT-V23P601

Atul Dev, Ajinder Kaur "Successful Propagation of Pigeonpea (Cajanus cajan L.) through In Vitro Shoot Regeneration and Ex Vitro Rooting", International Journal of Biotech Trends and Technology (IJBTT), V7(3): 1-6 Jul - Sep 2017, Published by Seventh Sense Research Group.

Abstract

A reproducible in vitro shoot regeneration protocol was developed for pigeonpea variety ICPL 87 through proliferation of axillary buds from cotyledonary nodes. The seedlings were cultured on MS + Kin (2 mgL-1) + BAP (5 mgL-1) medium and de-capitated on 7th day of culturing, which resulted in the induction of multiple shoots from cotyledonary node region after 15 days of culturing. Miniature shoots were elongated on MS + adenine suphate (30 mgL-1) medium; the elongated shoots were rooted ex vitro using a rooting powder ROOTEXTM.

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Keywords
Callus, Regeneration, Direct regeneration, Multiple shoots.