Isolation of Thermos table Extracellular Alkaline Protease and Lipase Producing Bacteria from Tannery Effluents

International Journal of Biotech Trends and Technology (IJBTT)
© 2015 by IJBTT Journal
Volume - 5 Issue - 1                         
Year of Publication : 2015
Authors : Udgire M., Vivek S., Sinha P.

Udgire M., Vivek S., Sinha P. "Isolation of Thermos table Extracellular Alkaline Protease and Lipase Producing Bacteria from Tannery Effluents", International Journal of Biotech Trends and Technology (IJBTT), V5(1):9-13 Jan - Mar 2015, Published by Seventh Sense Research Group.


The aim of the present study was to isolate the extracellular protease and lipase producing bacteria from tannery effluents. The bacterial isolation was performed by serial dilution and plating method. Total of eight protease producing isolates were screened on the basis of their clear zone formation on skim milk agar. Further screening for lipase producing stains on tributyrin agar confirmed that only four were able to produce an extracellular lipase enzyme. Isolated bacteria were identified on the basis of morphological, cultural, and biochemical characterization. Among four promising isolates three were belong to the genus Bacillus spp while one was identified as Actinobacillus spp.


1. Sapna, K., Sandeep Kumar and Jitender Kumar. Microbial decolourization of crystal violet by Bacillus subtilis. Biol. Forum Int. J. 3(1): 82-86, 2011.
2. R. Agrawal, R. Singh, A. Verma, P. Panwar, and A. K. Verma. World J. Agric. Sci. 8, 129 , 2012.
3. M. M. Altaf, F. Masood, and A. Malik, “Impact of long-term application of treated tannery effluents on the emergence of resistance traits in rhizobium sp. isolated from trifolium alexandrinum,” Turkish Journal of Biology, Vol. 32, pp. 1–8, 2008.
4. Ward O.P, “Enzyme Bioprocessing Applications” In Bioprocessing, edited by O.P Ward, (Open University Press, Buckingham, pp. 151-170, 1991.
5. Beisson F, Arondel V, Verger R. Assaying Arabidopsis lipase activity. BiochemSoc Trans, 28:773–5, 2000.
6. L. P. Andersen, Method for dehairing of hides or skins by means of enzymes. US Patent. 5, 834, 299, 1998.
7. G. Prabhavathy1, M. Rajasekara Pandian2 and B. SenthilkumarIdentification of Industrially Important Alkaline Protease Producing Bacillus subtilis by 16s rRNA Sequence Analysis and Its ApplicationsInternational Journal of Research in Pharmaceutical and Biomedical Sciences; Vol. 4 (1) Jan – Mar, 2013
8. Mukesh Kumar, D.J., Rejitha, R., Devika, S., Balakumaran M.D.A., Immaculate nancy rebecca and Kalaichelvan, P.T. Production, optimization and purification of lipase from Bacillus sp. MPTK 912 isolated from oil mill effluent. Adv. Appl. Sci. Res. 3(2): 930-938, 2012.
9. T. R. Srinivasan, S. Das, V. Balakrishnan, R. Philip, and N Kannan, Recent Res. Sci. Technol. 1, 63 ,2009.
10. K. Kanimozhi1, E.G. Wesely Jebasingh Devairrakam2 and D. Jegadeeshkumar3 ; Decolorization of Leather effluent by lipase producing Bacillus sp. J. Acad. Indus. Res. Vol. 1(12) May 2013 813
11. S. S. Yang and C. I. Huang, J. Chinese Agri. Chem. Soc. 32, 589 (1994).
12. Sangeetha, R., Geetha, A. and Arulpandi, I. Concomitant production of protease and lipase by Bacillus licheniformis vsg1: Production, purification and characterization. Brazilian J. Microbiol. 41: 179- 185, 2010.
13. R. F. Buchanan and N. F. Gibbons, Bergey’s Manual of Determinative Bacteriology, 9th edn., (Williums and Wilkins Co., Baltimore, USA, 1994.
14. Kambiz, M.H., Fereshteh Eftekhar, Bagher Yakhchaliand Fatemeh Tabandeh. Isolation and identification of a lipase producing Bacillus sp. from soil. Pak. J. Biol. Sci. 11: 740-745, 2008.
15. .E. Negrete-Abascal, S. V. Pacheco, G. L. Paniagua, A. P. Me´ndez, J. I. Caballero, V. M. Pe´rez Ma´rquez, and V. R. Tenorio, Cur. Microbiol. 49, 55, 2004.

Tannery Effluent; Bacillus; Actinobacillus; Extracellular Protease, Lipase