Isolation of Thermos table Extracellular Alkaline Protease and Lipase Producing Bacteria from Tannery Effluents

 
 
International Journal of Biotech Trends and Technology (IJBTT)
 
© 2015 by IJBTT Journal
Volume - 5 Issue - 1                         
Year of Publication : 2015
Authors : Udgire M., Vivek S., Sinha P.

Udgire M., Vivek S., Sinha P. "Isolation of Thermos table Extracellular Alkaline Protease and Lipase Producing Bacteria from Tannery Effluents", International Journal of Biotech Trends and Technology (IJBTT), V5(1):9-13 Jan - Mar 2015, Published by Seventh Sense Research Group.

Abstract

The aim of the present study was to isolate the extracellular protease and lipase producing bacteria from tannery effluents. The bacterial isolation was performed by serial dilution and plating method. Total of eight protease producing isolates were screened on the basis of their clear zone formation on skim milk agar. Further screening for lipase producing stains on tributyrin agar confirmed that only four were able to produce an extracellular lipase enzyme. Isolated bacteria were identified on the basis of morphological, cultural, and biochemical characterization. Among four promising isolates three were belong to the genus Bacillus spp while one was identified as Actinobacillus spp.

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Keywords
Tannery Effluent; Bacillus; Actinobacillus; Extracellular Protease, Lipase