IJBTT

Determination of The Influence of Used Engine Oil on Soil Microbial Community Around Mechanic Workshops

	International Journal of Biotech Trends and Technology (IJBTT)
	© 2019 by IJBTT Journal
	Volume - 9 Issue - 4
	Year of Publication : 2019
	Authors : Akubuenyi, F. C.
	DOI :  10.14445/22490183/IJBTT-V9I4P608

Citation

MLA Style:Akubuenyi, F. C.  Determination of The Influence of Used Engine Oil on Soil Microbial Community Around Mechanic Workshops International Journal of Biotech Trends and Technology 9.4 (2019): 52-58.

APA Style:Akubuenyi, F. C.(2019). Determination of The Influence of Used Engine Oil on Soil Microbial Community Around Mechanic Workshops  International Journal of Biotech Trends and Technology, 9(4),52-58.

Abstract

The influence of used engine oil on soil microbial community around a mechanic workshop was determined. Soil samples were collected from 5 different workshops, while the sample obtained from a nearby uncontaminated soil served as control. The Total Heterotrophic bacterial (THB) and fungal (TF) counts degreased throughout the study period, ranging from 3.4x105CFU/g to 5.22x104CFU/g and 8.7x104CFU/g to 4.20x103CFU/g respectfully. The characterization and identification of isolates revealed the presence of the following bacteria;Bacillus spp, Pseudomonas spp, Staphylococcus spp, Enterobacteriaspp, Micrococcus spp, Staphylococcus spp, Corynebacterspp, Lactobacillus spp and fungi;Mucor, Rhizopus, Penicillium, Fusarium and Aspergillus, as among the engine oil utilizing microorganisms.Bacillus species has the highest prevalence of 36.40%, followed by Staphylococcus spp with 18.18%. The analysis for utilization of the used engine oil by these organisms on a spectrophotometer revealed that the utilization as shown by the turbidity increased throughout the study period of 0hr to 144hrs, which showed that the microorganisms are utilizing the used engine oil, which indicates that they could be applied for remediation on an engine oil polluted soil.

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Keywords
Used engine oil, mechanic workshop, impacted soil, turbidity, engine oil utilizing bacteria