Determination of The Influence of Used Engine Oil on Soil Microbial Community Around Mechanic Workshops

International Journal of Biotech Trends and Technology (IJBTT)
© 2019 by IJBTT Journal
Volume - 9 Issue - 4                          
Year of Publication : 2019
Authors : Akubuenyi, F. C.
DOI :  10.14445/22490183/IJBTT-V9I4P608


MLA Style:Akubuenyi, F. C.  Determination of The Influence of Used Engine Oil on Soil Microbial Community Around Mechanic Workshops International Journal of Biotech Trends and Technology 9.4 (2019): 52-58.

APA Style:Akubuenyi, F. C.(2019). Determination of The Influence of Used Engine Oil on Soil Microbial Community Around Mechanic Workshops  International Journal of Biotech Trends and Technology, 9(4),52-58.


The influence of used engine oil on soil microbial community around a mechanic workshop was determined. Soil samples were collected from 5 different workshops, while the sample obtained from a nearby uncontaminated soil served as control. The Total Heterotrophic bacterial (THB) and fungal (TF) counts degreased throughout the study period, ranging from 3.4x105CFU/g to 5.22x104CFU/g and 8.7x104CFU/g to 4.20x103CFU/g respectfully. The characterization and identification of isolates revealed the presence of the following bacteria;Bacillus spp, Pseudomonas spp, Staphylococcus spp, Enterobacteriaspp, Micrococcus spp, Staphylococcus spp, Corynebacterspp, Lactobacillus spp and fungi;Mucor, Rhizopus, Penicillium, Fusarium and Aspergillus, as among the engine oil utilizing microorganisms.Bacillus species has the highest prevalence of 36.40%, followed by Staphylococcus spp with 18.18%. The analysis for utilization of the used engine oil by these organisms on a spectrophotometer revealed that the utilization as shown by the turbidity increased throughout the study period of 0hr to 144hrs, which showed that the microorganisms are utilizing the used engine oil, which indicates that they could be applied for remediation on an engine oil polluted soil.


[1] C. S. Butler, J. R. Mason, Structure– function Analysis of the Bacterial Aromatic ring–hydroxylatingDioxygenases. Advanced Microbial Physiology, vol. 38, pp 47–84, 1997.
[2] K.A. Kvenvolden, Organic Geochemistry – A retrospective of its first 70 years.Organic Geochemistry, 37, 1, 2006.
[3] P.K. Jain, V.K. Gupta, R.K. Gaur, M. Lowry, D.P. Jaroli,U.K. Chauhan, Bioremediation of petroleum oil contaminated soil and water. Research Journal of environmental Toxicology, vol. 5, no. 1, 1-26, 2009.
[4] A.O. Adeleye, M.E. Nkereuwem, G.I. Omokhudu, A. Amoo, G.P. Shiaka, M.B. Yerima, Effect of microorganisms in the bioremediation of spent engine oil and petroleum related environmental pollution, vol. 22 no: 2 pp 157 – 167, 2018.
[5] S. Brussels, R. Luxembourg, Recycling of used mineral oil. Journals of Mechanical Engineering and Processing Technology, vol. 8, no. 12, pp 67-81, 2011.
[6] S.O. Ogbeide, An Investigation to the Recycling Of Spent Engine Oil. Journal of Engineering Science and Technology Review, vol. 3, no. 1, pp 32-35, 2010.
[7] Agency for Toxic Substances and Disease Registry (ATSDR), Toxicology profile for used mineral base crankcase oil. Department of Health and Human Services, Public Health service Press, Atlanta, GA, USA, 1997.
[8] M. Mohd, S. Shiv, M.Y. Shikha, R.N. Shukai, Remediation of hydrocarbon contaminated soil through microbial degradation – FTIR based prediction. Advances in Applied Science Research, vol. 2, no. 2, pp 321 – 326, 2011.
[9] O.P. Abioye, P. Agamuthu, A.R. Abdul-Aziz, “Biodegradation of used motor oil in soil usin0g organic waste amendments”. Biotechnology Resource Institute.pp 140-145. 2012
[10] U. I. Uchendu, P. A. Ogwo, The effect of spent engine oil discharge on soil properties in an automobile mechanic village in Nekede, Imo State, Nigeria, vol. 8, pp 28-32, 2014.
[11] G.O. Anoliefo, D.E. Vwioko, Tolerance of Chromolaenaodorata (L) grown in soil contaminated with spent lubrication oil. Journal of Tropical Biosciences, vol. 1, pp 20-24, 2010.
[12] C. Chinenye, O. Chijioke, Effects of waste engine oil on the microfloraand physico-chemical quality of soils from two West African countries. Asian Journal of Environment & Ecology, vol. 1, no. 2, pp 1-14, 2016.
[13] S.C. Ugoh, L.U. Moneke, Isolation of bacteria from engine oil contaminated soils in Auto mechanic workshops in Gwagwalada, Abuja, FCT-Nigeria. Academia Arena, vol. 3, no. 5, pp 28-33, 2011.
[14] V.J. Odjegba, A.O. Sadiq, Effect of spent oil on the growth parameters, chlorophyll and protein levels of amaranthushybidus L. The Environmentalist, vol. 22, pp 23-28, 2002.
[15] G.O. Anoliefo, B.O. Edegbai, Effect of spent engine oil as a soil contaminant on the growth of two egg plant species, Solanummelongena and Solanumincanum L. Journal of Agriculture, Forestry and Fisheries, vol.1, pp 21-25, 2012.
[16] M.R. Smith, The biodegradation of aromatic hydrocarbon by bacteria. Biodegreaded,vol. 1 pp 191-206, 2016.
[17] M.M. Stancu, Bacterial Degradation of Petroleum and Petroleum Products. Journal of Molecular Microbiology, vol. 2, no. 1, p 2, 2018.
[18] R.M. Ebenezer, Biological degradation of petroleum hydrocarbon: An environmental perspective. Microbiology Review.Vol. 45, pp 180-200, 2016.
[19] C. O. Jesubunmi, Isolation of Oil – Degrading microorganisms in spent engine oil – contaminated soil. Journal of Biology, Agriculture and Healthcare, vol. 4, no. 24,pp 191-195, 2014.
[20] M.Cheesbrough, District Laboratory Practice in Tropical Countries. Part 2.Cambrigde University Press. pp. 105-112, 2002
[21] A. Samuel, W. Wemedo, O. Janet, J. C. Odebe, Seasonal influence on Microbial load in waste engine oil polluted soil of automechanic workshop in Port Harcourt metropolis. IOSR Journal of Environmental Science, Toxicology and Food Technology, vol. 13 no. 3, pp 54-60, 2019.
[22] L. O. Odukuma, C. Osuagwu, Tolerance of chemolithotrophic bacteria to organichlorine, organophosphate and contaminant pesticide.Journal of Agriculture and Environmental Engineering Tecnology, vol.1, no. 1, pp 7-15, 2004.
[23] L. O. Okpokwasili, A.I. Nwosu, Degradation of Aldim by bacterial isolates. Nigeria Journal of Technology Research, vol. 2, pp1-6, 2010.
[24] O.I. Enabulele, O.N. Obayagbona, Biodegradation Potentials of Mycoflora Isolated from Auto Mobile Workshop Soils on Flow Station Crude Oil Sludge. International Research Journal of Biological Sciences, vol. 2, pp 5, pp 9 – 18, 2013.
[25] S. O. Oluwafemi, O. I. Matthew, A. A. Sunday, O. A. Olukayode, O. O. Ganiyu, Microbial Population Changes in Tropical Agricultural Soil Experimentally Contaminated with Crude Petroleum. African Journal of Biotechnology, vol. 7, no.24, pp 4512-4520, 2008.
[26] B.E. Asikong, R.U.B. Ebana, Hydrocarbon degraders as indicates of environmental pollution by petrol stations in Calabar internet. Journal tropical Environment, vol. 2, pp167-178, 2010.
[27] P. G. Olannye, K. I. Ogru, A. O. Emoghene, Remediation of used engine oil contaminated soil by fungal isolates from mechanic workshop soils. Nigerian Journal of Microbiology, vol. 31, no, 1, pp 3807-3816, 2017.
[28] B.O. Okonokua, B. Ikhajiagbe, G.O. Anoliefo, T. O. Emede, The Effects of Spent Engine Oil on Soil Properties and Growth of Maize (Zea mays L.) J. Applied Science Environmental Management. September, vol 11, no. 3, pp 147 – 152, 2007.
[29] M.B. Adewole, L.U. Uchegbu, Properties of soils and plants uptake within the vicinity of selected automobile workshops in Ile-Ife Southwestern, Nigeria. Ethiopian Journal of Environmental Studies Management.vol. 3, pp 3, 2010.
[30] J. Wyszkowski, J. Kucharski, E. Jastrzebska, A. Hlasko, The biological properties of soil as influenced by chromium contamination. Polish Journal of Environmental Studies, vol.10, pp 37-42, 2018.
[31] A.U. Nkwoada, C.O. Alisa, C.M. Amakom, Pollution in Nigerian auto-mechanic villages: A Review. IOSR Journal of Environmental Science, Toxicology and Food Technology, vol.12, no. 7, pp 43-54, 2018.

Used engine oil, mechanic workshop, impacted soil, turbidity, engine oil utilizing bacteria