Isolation, Characterization And Optimization Of Amylase Producing Organisms From Soil of Sabarkantha District

 
 
International Journal of Biotech Trends and Technology (IJBTT)
 
© 2019 by IJBTT Journal
Volume - 9 Issue - 4                          
Year of Publication : 2019
Authors : Jagruti Patel,Dhaval Prajapati, Vikram Solanki, Shreyas Bhatt
DOI :  10.14445/22490183/IJBTT-V9I4P607

Citation

MLA Style:Jagruti Patel,Dhaval Prajapati, Vikram Solanki, Shreyas Bhatt  Isolation, Characterization And Optimization Of Amylase Producing Organisms From Soil of Sabarkantha District International Journal of Biotech Trends and Technology 9.4 (2019): 48-51.

APA Style:Jagruti Patel,Dhaval Prajapati, Vikram Solanki, Shreyas Bhatt(2019).Isolation, Characterization And Optimization Of Amylase Producing Organisms From Soil of Sabarkantha District  International Journal of Biotech Trends and Technology, 9(4),48-51.

Abstract

Amylases are amongst most widely used enzymes in industries such as food, fermentation, starch processing, textile and paper. In the present investigation, bacteria were isolated from Garden a farm site of different regions of the Idar Sabarkantha District, screened for the production of amylase and their optimum growth conditions were determined. A total 10 bacterial colonies were isolated from collecting soil samples. Ten bacterial isolates, displayed zones of clearance in the starch hydrolysis test. The isolate displaying maximum amylase activity on the constitution was selected. A characteristic feature of the strain indicates that it belongs to the genus Bacillus and streptomycin and will be later used for further characterization. Maximum yield of amylase was obtained after 48hrs of incubation. The optimum pH, carbon source, Nitrogern Source,Salt, Temperature,Shaking seed and Growth Factor for enzyme activity.

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Keywords
Bacillus,streptomycin, starch hydrolysis,carbon source,Nitrogen Source, optimum temperature