Isolation, Characterization And Optimization Of Amylase Producing Organisms From Soil of Sabarkantha District
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International Journal of Biotech Trends and Technology (IJBTT) |  |
| © 2019 by IJBTT Journal | ||
| Volume - 9 Issue - 4 |
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| Year of Publication : 2019 | ||
| Authors : Jagruti Patel,Dhaval Prajapati, Vikram Solanki, Shreyas Bhatt | ||
| DOI : 10.14445/22490183/IJBTT-V9I4P607 |
Citation
MLA Style:Jagruti Patel,Dhaval Prajapati, Vikram Solanki, Shreyas Bhatt Isolation, Characterization And Optimization Of Amylase Producing Organisms From Soil of Sabarkantha District International Journal of Biotech Trends and Technology 9.4 (2019): 48-51.
APA Style:Jagruti Patel,Dhaval Prajapati, Vikram Solanki, Shreyas Bhatt(2019).Isolation, Characterization And Optimization Of Amylase Producing Organisms From Soil of Sabarkantha District International Journal of Biotech Trends and Technology, 9(4),48-51.
Abstract
Amylases are amongst most widely used enzymes in industries such as food, fermentation, starch processing, textile and paper. In the present investigation, bacteria were isolated from Garden a farm site of different regions of the Idar Sabarkantha District, screened for the production of amylase and their optimum growth conditions were determined. A total 10 bacterial colonies were isolated from collecting soil samples. Ten bacterial isolates, displayed zones of clearance in the starch hydrolysis test. The isolate displaying maximum amylase activity on the constitution was selected. A characteristic feature of the strain indicates that it belongs to the genus Bacillus and streptomycin and will be later used for further characterization. Maximum yield of amylase was obtained after 48hrs of incubation. The optimum pH, carbon source, Nitrogern Source,Salt, Temperature,Shaking seed and Growth Factor for enzyme activity.
References
[1] Alariya, S. S., Sethi, S., Gupta, S., & Lal, G. B. (2013). Amylase activity of a starch degrading bacteria isolated from soil. Archives of applied science Research, 5(1), 15- 24.
[2] Alariya, S. S., Sethi, S., Gupta, S., & Lal, G. B. (2013). Amylase activity of a starch degrading bacteria isolated from soil. Archives of applied science Research, 5(1), 15- 24.
[3] Aqeel, B. M., & Umar, D. M. (2010). Effect of alternative carbon and nitrogen sources on production of alpha-amylase by Bacillus megaterium. World Applied Sciences Journal, 8(13), 85-90.
[4] Bala, J. D., Abioye, O. P., Auta, H. S., Damisa, D., Adabara, N. U., & Tuggen, T. O. (2013). Screening of soil microorganisms for amylase production.
[5] Bernfeld P, Methods Enzymol, 1955, 1: 149-158
[6] Chopra, P. (2006, January). Drought risk assessment using remote sensing and GIS: a case study of Gujarat. ITC.
[7] Dick, R. P., & Kandeler, E. (2005). Enzymes in soils.
[8] Gebreyohannes, G. (2015). Isolation and optimization of amylase producing bacteria and actinomycetes from soil samples of Maraki and Tewedros campus, University of Gondar, North West Ethiopia. African Journal of Microbiology Research, 9(31), 1877-1882.
[9] Gopinath, S. C., Anbu, P., Arshad, M. K., Lakshmipriya, T., Voon, C. H., Hashim, U., & Chinni, S. V. (2017). Biotechnological processes in microbial amylase production. BioMed research international, 2017.
[10] Jung, D. H. (1980). Preparation and application of procion yellow starch for amylase assay. Clinica Chimica Acta, 100(1), 7-11.
[11] Manimaran, M., & Kannabiran, K. (2018). Alpha amylase activity of Streptomyces sp. VITMK1 isolated from marine soil sample of Pichavaram, Tamil Nadu, India. Research Journal of Pharmacy and Technology, 11(6), 2180-2182.
[12] Patel, A. C., Patel, A. K., Pavaya, R. P., & Patel, V. R. (2017). Status of available major nutrients in soils of Sabarkantha district of Gujarat. IJCS, 5(6), 1326-1329.
[13] Singh, P., & Kumari, P. (2016). Isolation and characterization of amylase producing Bacillus spp. from selected soil sample. International Journal of Research in Biosciences, 5(2), 24-29.
Keywords
Bacillus,streptomycin, starch hydrolysis,carbon source,Nitrogen Source, optimum temperature