Genetic Diversity of Rhizobial isolates revealed by PCR-RAPD fingerprinting and the data were analyzed using POPGEN

Genetic Diversity of Rhizobial isolates revealed by PCR-RAPD fingerprinting and the data were analyzed using POPGEN.

 
 		 International Journal of Biotech Trends and Technology (IJBTT)
 
© 2019 by IJBTT Journal
Volume - 9 Issue - 4                          
Year of Publication : 2019
Authors : Rajasekaran. R, Chandrasekaran. R, Muthuselvam. M, Arunkumar. S, Senthil kumar. S
DOI :  10.14445/22490183/IJBTT-V9I4P605

Citation

MLA Style:Rajasekaran. R, Chandrasekaran. R, Muthuselvam. M, Arunkumar. S, Senthil kumar. S  "Genetic Diversity of Rhizobial isolates revealed by PCR-RAPD fingerprinting and the data were analyzed using POPGEN." International Journal of Biotech Trends and Technology 9.4 (2019): 18-41.

APA Style:Rajasekaran. R, Chandrasekaran. R, Muthuselvam. M, Arunkumar. S, Senthil kumar. S (2019). Genetic Diversity of Rhizobial isolates revealed by PCR-RAPD fingerprinting and the data were analyzed using POPGEN. International Journal of Biotech Trends and Technology, 9(4),18-41.

Abstract

Rhizobia are soil bacteria which specifically nodulate legume roots thus forming a nitrogen fixing root nodule symbiosis, which has a great importance to agriculture in nitrogen deficient environments. The study aimed at investigating the genetic diversity of fourteen different rhizobial isolates using PCR-RAPD fingerprinting and the data were analyzed using POPGEN. Total genomic DNAs from different field isolates were amplified using five different arbitrary primers (OPA02, OPA03, OPM17, OPP07 and OPP08), the amplified DNA solution was subjected to agarose gel electrophoresis. Around 600 different bands (558 polymorphic) were produced from 14 isolates showing 93% of overall polymorphism. All the five primers produced polymorphic bands with OPA03 and OPP08 registering one hundred per cent polymorphic bands. Among these two, only OPP08 produced unique bands (2.30%). The percentage of monomorphic bands produced by OPA02, OPM17 and OPP07 were 9.65, 16.66 and 17.07 respectively. Similarly the percentages of unique bands for the above primers were 2.06, 1.19 and 2.43. Resolving power (Rp) of the primers ranged from 1.84 (OPM17) through 1.86 (OPA03, OPP07, OPP08) to 1.90 (OPA02). Summary of genetic variation statistics for all loci produced by five random primers using POPGEN version 1.31software reveal that primer OPA03 is more effective in highlighting the variation between the isolates. Nei’s unbiased measures of genetic identity and genetic distance were used to construct a similarity matrix and a rectangular cladogram was constructed based on UPGMA analysis. Ewans-Watterson test for neutrality confirms the existence of genetic diversity in all the polymorphic loci indicating their neutral nature with regard to evolution of the isolates.

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Keywords
Rhizobia, DNA profiles, RAPD, Genetic diversity, POPGEN.