IJBTT

Metagenomics of Bio Slurry Obtained From A Biogas Plant Using Next-Generation Dna Sequencing

	International Journal of Biotech Trends and Technology (IJBTT)
	© 2019 by IJBTT Journal
	Volume - 9 Issue - 3
	Year of Publication : 2019
	Authors : Akubuenyi F.C, Odokuma, L.O., Nwaokorie, F.O.
	DOI :  10.14445/22490183/IJBTT-V9I3P603

Citation

MLA Style:Akubuenyi F.C, Odokuma, L.O., Nwaokorie, F.O. "Metagenomics of Bio Slurry Obtained From A Biogas Plant Using Next-Generation Dna Sequencing" International Journal of Biotech Trends and Technology 9.2 (2019): 12-17.

APA Style:Akubuenyi F.C, Odokuma, L.O., Nwaokorie, F.O.(2019). Metagenomics of Bio Slurry Obtained From A Biogas Plant Using Next-Generation Dna Sequencing. International Journal of Biotech Trends and Technology, 9(2), 12-17.

Abstract

Metagenomics of bioslurry obtained from biogas plant was carried out Next-Generation DNA sequencing. The DNA of the associated bacterial organisms was extracted using a ZYMO Research DNA extraction kit (Quick-gDNATMMiniPrep). They were sequenced by Next Generation Sequencing Technique to determine the nucleotide sequence of all microorganisms present in the sample using automated PCR cycle- Genome Sequencer™ FLX System from 454 Life Sciences™ and Roche Applied.Sequence analysis and alignment was performed using Vecton NTI suite 9 (InforMax, Inc.). The partial 16S rRNA gene sequences generated and subjected to BLAST analysis, and compared to GenBank database of the National Center for Biotechnology Information revealed the presence of Clostridium thermocellum, Clostridium tetani, Spirochaeta caldaria, Acinetobacterbaumanni, Stenotrophomonasmaltophilic, Prevotellaruminocola, Parabacteriumdistasonics, Clostridium cellulovorans, Mahellaaustraliensis, Ethanoligenensharbinense, Odoribactersplanchnicus, Tannerallaforsythis, Clostridium stercorarium, Gramellaforsetti, GeobacillusthermoleovoransHalibacteriummodesticaldum and Veillonellaparvula, as the bacteria involved in biogas production. This implies that biogas production is mediated by these species of anaerobic bacteria.

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Keywords
Metagenomics, Bioslurry, Biogas plant, Next generation DNA sequencing