Comparative Studies of Callus Cell and Shoot Proliferation From Pineapple And Banana Culture in Vitro: Antioxidant, Carbohydrate, Pigment and Mineal Properties

International Journal of Biotech Trends and Technology (IJBTT)
© 2018 by IJBTT Journal
Volume - 8 Issue - 2                          
Year of Publication : 2018
Authors : ABM Sharif Hossain
DOI :  10.14445/22490183/IJBTT-V8I2P601


ABM Sharif Hossain "Comparative Studies of Callus Cell and Shoot Proliferation From Pineapple And Banana Culture in Vitro: Antioxidant, Carbohydrate, Pigment and Mineal Properties", International Journal of Biotech Trends and Technology (IJBTT), V8(2): 1-6 April - June 2018, Published by Seventh Sense Research Group.


The study was conducted to evaluate the comparison of the callus cell, shoot and stem proliferation from pineapple crown slice and banana inflorescence in vitro using NAA and BAP at different concentration. The pineapple shoot number and weight was higher in BAP at 2.0 mg/l than control and NAA 0.2 mg/l. However, the BAP + IAA (10mg/l + 1 mg/l) concentration produced higher callus cell weight, shoot number and stem length than MS medium (without hormone) and NAA 1mg/l concentrations in banana. Moreover, it has been shown that the sugar and chlorophyll content were higher in BAP treated hormone than MS medium and NAA 0.2mg/l concentration in pineapple. In addition to that the nutrient content (K+ and NO3--) was exhibited higher in the 2 mg/l BAP treated explants than MS (control) and 0.2 mg/l NAA treated explants in pineapple. Callus tissue weight was maximal in BAP (10mg/l) + IAA (1mg.l) treated explants compared to the NAA (1 mg/l) and MS treated explants in banana. Besides, shoot number and stem length were higher in the BAP (10mg/l) + IAA (1mg/l) treated explants than NAA (1 mg/l) and MS (control) media in banana. The highest sugar and chlorophyll content was recorded in BAP (10mg/l) + IAA (1mg/l) treated explants in banana. Meanwhile, the total phenol content was significantly increased by BAP treatment. K+ and NO3-- content in explants were significantly increased by BAP and NAA treatments in banana. The result showed that the DPPH radical scavenging activity increased with hormone application. Finally it seemed that BAP was the best effective hormone for the both pineapple and banana explants regeneration. Therefore the highest shoot and stem was regenerated in BAP (2mg/l) in pineapple.


1. A.B.M.S. Hossain, Plant Cell and Tissue culture Biotechnology :Stem cell. LAP Lambart Academic publishing Co. Paperback, Germany. ISBN No. 9783659595943 Pp216.2014.
2. C. Zepeda, Y. Sagawa, In vitro propagation of pineapple. Hortic. Sci., 16: 495-495.1981.
3. L.J., Liu, E. Rosa-Marquez, E. Lazardi, Smooth leaf (spineless) red spanish pineapple (Ananas comosus (L.) Merr) propagated in vitro. J. Agric. Univ. 1989.
4. S., R. Sripaoraya, J.B. Marchant, and M.R. Davey, Plant regeneration by somatic embryogenesis and organogenesis in commercial pineapple (Ananas comosus L.). In Vitro Cell Dev. Biol. Plant, 39: 450-454. 2003.
5. A.B.M.S. Hossain Innovative Plant Biotechnology and Molecular Biology. LAP Lambart Academic publishing Co. Paperback, Germany. ISBN No. 9783659320583 Pp179.2013.
6. W. Sani., A.B.M.S. Hossain, S. Chandran, Plantlet Production through Development of Competent Multiple Meristem Cultures from Male Inflorescence of Banana, Musa acuminta cv. Pisang Mas (AA). American Journal of Biochemistry and Biotechnology 4(4): 325-328. 2008.
7. Y.W., Ho, Y.P. Tan, C. Mak, Micro propagated for commercial production of planting materials with special reference to banana. Proceedings of Seminar on the Fruit Industry in Malaysia, September 7-9, Johor Bharu, Malaysia. Pp 14-19. 1993.
8. R., Morpurgo, S.V. Nto, R. Afza, F.J. Novak, Selection parameters for resistance to Fusarium oxysporum f. sp. cubense race 1 and race 4 on diploid banana (Musa acuminata Colla). Euphtica, 75: 121-129. DOI 10.1007/BF00024539/ISSN 014- 2336 (Print) 1573-5060. 1994.
9. SS. Cronauer, A, Krikorian Aseptic multiplication of banana from excised floral apices. Horticulture,20: 770-771. ISSN 0018- 5345 27. Aydieh A.A, Ibrahim M.K.H, Ibrahim A, 2000. In vitro propagation and fruiting of pineapple. Egypt. J. Hortic., 27: 289-304. 1988.
10. J.V Escalant, C., Teisson Somatic embryogenesis from immature zygotic embryos of the species. Musa acuminata and Musa balbisiana. Plant Cell Rep., 7: 665-668. DOI : 10.1007/BF00272056/ ISSN 0721-7714 (Print) 1432-203X. 1989
11. S.S. Ma, Somatic embryogenesis and plant regeneration from cell suspension culture of banana. Proceeding of Symposium on Tissue Culture of Horticulture Crop, March 8-9, Taipei, Taiwan, pp: 181-188. 1991.
12. J.V Escalant,., C. Teisson, F. Cote, Amplifield somatic embryogenesis from male flowers of triploid banana and plantain cultivars (Musa spp). In vitro Cell Dev. Biol., 30: 181-186. DOI: 10.1007/BF02823029/ISSN : 1054-5476 (Print) 1475-2689. 1994.
13. J., Mahanom, N. Khalid and R.Y Othman, Plant regeneration from embryogenic suspension cultures of Musa acuminate cv. Mas (AA). Plant Cell Tiss. Organ Cult., 75: 209- 214. DOI : 10.1023/A:1025814922547 /ISSN : 0167-6857 (Print) 1573-5044. 2003.
14. R.H. Stover, N.W. Sidmmonds, Banana. In :Longman Scientific and technical (eds) Group Limited, London. Pp 448- 477. 1987.
15. A., Grapin, J.L. Ortiz, T. Lescot, N. Ferriere and F.X. Cote, Recovery and regeneration of embryogenesis cultures from female flowers of False Horn Plantain (Musa AAB). Plant Cell Tiss. Organ Cult., 61: 237-244. DOI : 10.1023/A:1006423304033/ISSN : 0167-6857 (Print) 1573-5044. 2000.
16. L. Resmi, and A.S. Nair, Plantlet production from the male inflorescence tips of Musa acuminata cultivars from South India. Plant Cell Tiss. Organ Cult. , 88: 333-338. DOI: 10.1007/s11240- 007-9206-7. 2007.
17. M.K,. Dubois, J.K. Gils, PA, Hanniton S. F., and Robes. Use of phenol reagent for the determination of total sugar,” Analytical Chemistry, vol. 28, pp. 350–354, 1956. 2008.“
18. E., Firoozabady, N., Gutterson Cost effective in vitro propagation methods for pineapple. Plant Cell Rept., 21: 844- 850.17. Be, LV, Debergh PC. 2006. Potential low cost micropropagation of pineapple (Ananas comosus). S. Afr. J. Bot. 72: 191-194. 2003.
19. P Boxus J.M., Terzi, C, Lieves, M. Pylyser, P, Ngaboyamahina, K., Duhem Improvement and perspectives of micropropagation techniques applied to some hot climate plants. Acta Hortic. 289: 55-59. 1991.
20. B. Yang, M. Zhao, J. Shi, N. Yang, and Y. Jiang,.“Effect of ultrasonic treatment on the recovery and DPPH radical scavenging activity of polysaccharides from longan fruit pericarp,” Food Chemistry, vol. 106, no. 2, pp. 685–690. 2008
21. S.L., Teixeira, J.M. Ribeeiro and M.T. Teixeira, Influence of NaCIO on nutrient medium sterilization and on pineapple (Ananas comosus cv. Smooth cayenne) behavior. Plant Cell Tiss. Organ Cult., 86: 375-378. 2006.
22. J.R., Soneji, P.S. Rao and M. Mhatre, Somaclonal variation in micropropagated dormant axillary buds of pineapple (Ananas comosus L. Merr.). J. Hort. Sci. Biotechnol., 77: 28-32. 2002.
23. T. Murashige, and F. Skoog, A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant., 15: 473-497.Cronauer, S.S. and A.D. 1962.
24. A.M. Hamad, and R.M. Taha, The effect of different hormones and incubation periods on in vitro proliferation of pineapple (Ananas comosus L.) Merr cv. Smooth Cayenne) shoottip culture. Pak. J. Biol. Sci., 11: 386-391. 2009.
25. Krikorian, M. Plant generation via somatic embryogenesis in the seeded diploid banana Musa ornata Roxb. Plant Cell Rep., 7: 23-25. DOI : 10.1007/BF00272970/ISSN 0721-7714 (Print) 1432-203x. 1988.
26. H.K., Lichtenthaler, A.R., Wellburn, Determinations of total carotenoids and chlorophylls a and b of leaf extracts in different solvents. Biochem. Soc. Trans. 11, 591–592. 1983.

callus cell and tissue, proliferation, pineapple crown and banana bud, antioxidant