In Vitro Micro Tuber Formation in Potato (Solanum tuberosum L.): is there any Relation between Methyl Jasmonate, Sugars, and Explants?

International Journal of Biotech Trends and Technology (IJBTT)
© 2018 by IJBTT Journal
Volume - 8 Issue - 1                          
Year of Publication : 2018
Authors : Gülsün E. Vural, Tugce Ozsan, Volkan Gozen, Ahmet N. Onus
DOI :  10.14445/22490183/IJBTT-V8I1P601


Gülsün E. Vural, Tugce Ozsan, Volkan Gozen, Ahmet N. Onus "In Vitro Micro Tuber Formation in Potato (Solanum tuberosum L.): is there any Relation between Methyl Jasmonate, Sugars, and Explants?", International Journal of Biotech Trends and Technology (IJBTT), V8(1): 1-8 January - March 2018, Published by Seventh Sense Research Group.


Studies on potato (Solanum tuberosum L.) plants have been carried out to plant tissue culture laboratories because of the disease and virulent free seed tubers in field conditions. Since generative production in commercial potato production is not economically massive, production is made more economically with tubers. Jasmonic acid (JA) and its related compounds are newly recognized plant growth regulators and they are known to have effects on many physiological processes. In this study, it was aimed to determine the effects of methyl jasmonate and sucrose on micro tuber formation in potato micropropagation. To serve the purpose the relationships between twelve media combinations (including control medium), varying concentrations of sucrose (3, 6 and 9 %) either alone or in combination with 1.0 ppm or 2.0 ppm methyl jasmonate were investigated. Three different plant parts such as node, shoot eye and primordial tissue wereused as explant sources. Nitsch&Nitsch was used as the basic nutrient medium and plant growth regulators that used in media were constant at 2.5 mg/L BAP + 0.5 mg/L NAA except for control medium. Obtained micro tubers, plantlets and callus formations were recorded and analyzed. Results revealed that, when methyl jasmonate used at 1.0 ppm concentration in media, it had a positive effect on micro tuber formation and can be recommended to use commercially in mass production.


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Solanum tuberosumL., micro tuber, methyl jasmonate, sucrose, in vitro.