Development of Modified Medium for Enhanced Production of Lipase by Streptomyces Halstedii Strain ST 70 Obtained From Bhitarkanika Mangroves

International Journal of Biotech Trends and Technology (IJBTT)
© 2014 by IJBTT Journal
Volume - 4 Issue - 4                          
Year of Publication : 2014
Authors : Srilekha Mishra , Nibha Gupta


Srilekha Mishra , Nibha Gupta. "Development of Modified Medium for Enhanced Production of Lipase by Streptomyces Halstedii Strain ST 70 Obtained From Bhitarkanika Mangroves", International Journal of Biotech Trends and Technology (IJBTT), V4(4):1-7 Oct - Dec 2014, Published by Seventh Sense Research Group.


The lipase production by Streptomyces halstedii strain ST 70, a mangrove isolate was enhanced through the modifications of cultural conditions and nutritional amendments. The strain produced 26.1 U/mL enzyme in presence of starch, glycine and ammonium chloride that was enhanced upto 56.1 U/ml with 50% ammonium sulphate precipitation. Results showed that tween 80 is required as inducer to enhance the lipase production. The enzyme was found to be stable at various temperature and pH where as saline condition did not favour higher enzyme activity. The present study reveals importance towards the industrial level exploitation of this strain due to its origin and only few Streptomyces strains are reported for lipase production.


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Lipase, Streptomyces, mangrove, nutrient, carbon source, nitrogen source