Use of RAPD and ISSR Markers for Molecular Genetic Analysis of Eucalyptus tereticornis

International Journal of Biotech Trends and Technology (IJBTT)
© 2014 by IJBTT Journal
Volume - 4 Issue - 3 
Year of Publication : 2014
Authors : Devendra Nishad , Priya Singh , Sujeet K. Singh


Devendra Nishad , Priya Singh , Sujeet K. Singh. "Use of RAPD and ISSR Markers for Molecular Genetic Analysis of Eucalyptus tereticornis", International Journal of Biotech Trends and Technology (IJBTT), V4(3):31-33 Jul - Sep 2014, Published by Seventh Sense Research Group.


Random Amplified Polymorphic DNA (RAPD) and Inter Simple Sequence Repeat (ISSR) polymorphism was employed to assess the genetic variations in the germplasm of Eucalyptus tereticornis. 15 trees under cultivation were analysed with 10 RAPD primers and 4 ISSR Primers of which all RAPD Primers and only 2 ISSR primers showed reproducible results. Marker attributes like Polymorphism Information Content (PIC), Effective Multiplex Ratio (EMR), and Marker Index (MI) and Informative bands (Ib) values were calculated to assess the discriminatory power of RAPD and ISSR primers. For RAPD primers the PIC values ranged from 0.34 to 0.50, the EMR ranged from 34 to 201, the MI value ranged from 17 to 88.44 with an average of 9.119 per primers. For ISSR primers the PIC values ranged from 0.36 to 0.41 with an average of 0.38 per primer. The EMR ranged from 122 to 129.6 and the MI values ranged from 50.02 to 46.65 with an average of 46.65 per primer. The Ib value ranged from 50 to 56 with an average of 53 per primer combination. The UPGMA-phenogram categorized the 15 trees into two major clusters based on genetic similarity and dissimilarity.


[1] Lal, P., Kulkarni, H.D., & Srinivasa, K., Venkatesh, K.R. & Sanhakumar, P. 1997. Genetically improved clonal planting stock of Eucalyptus – A success story from India. Indian Forester 123: 1117-1138.
[2] Purkayastha, S.K., Agrawal., S.P.,Farooqui, P., Tandon, R.D., Laxmi Chauhan & Neelam, M. 1979. Evaluation of Wood Quality of Eucalyptus Plantations in Various States. Final Technical Report of Project No. Inn FS-66, Forest Research Institute, Dehra Dun. 85 pp.
[3] Sharma, Y.K. & Bhandari, K.S. 1983. Eucalyptus for pulp and paper making. Indian Forester 109: 944-950.
[4] Bhat, K.M. 1990. Wood quality improvement of eucalypts in India. An assessment of property variations. Journal of the Indian Academy of Wood Science 21(2): 33-38.
[5] Bhat, K.M. & Bhat, K.V. 1984. Wood properties of 1-year-old Eucalyptus tereticornis Sm. Australian Forest Research 14:129-133.
[6] Bhat, K.M., Dhamodaran, T.K.Bhat, K.V. 7 Thulsidas, P.K. 1987. Wood property variation of 3-year-old trees among four eucalypt species grown in Kerala. Journal of the Indian Academy of Wood Science 18(2): 7-12.
[7] Singh, S.V. & Naithani, S. 1994. Pulpwood demand and quality assessment. IPPTA Convention Issue 99-111. Indian Pulp and Paper Technical Association, Saharanpur.
[8] Rao, R.V., Shashikala, S., Sreevani, P.,Vimal Kothiyal, Sharma, G.R. & Piare Lal 2002. Within tree variation in anatomical properties of some clones of Eucalyptus tereticornis Sm. Wood Science and Technology 36(3): 271-285.
[9] J Welsh and M McClelland.1990. Fingerprinting genomes using PCR with arbitrary primers. Nucleic Acids Res.18(24): 7213–7218.
[10] Carlson, J. E.; Tulsiearam, L. K.; Glaubita, J. C.; Luck, V. W. K.; C. and Rutedge, R. (1991). Segregation of random amplified DNA markers in F1 progeny of conifers. Theor. Appl. Gen. 83: 194-200.
[11] Skabo, S., Vaillancourt, R. E., Potts, B. M. (1998). Fine scale genetic structure of Eucalyptus globules spp. Globulus forest revealed by RAPDs. Australian Journal of Botany 46(5/6):583-594.
[12] Stange, C., Prehn, D., Jhonson, P. A. (1998). Isolation of Pinus radiata Genomic DNA suitable for RAPD analysis. Plant Molecular Biology Reporter16:1-8.

RAPD marker, UPGMA clustering, Polymerase Chain Reaction, EMR, PIC, MI and ISSR Markers.