In Vitro Detection of Uropathogenic Bacteria from UTI Infected Patients and Their Suitable Drugs Selection

International Journal of Biotech Trends and Technology (IJBTT)
© 2014 by IJBTT Journal
Volume - 4 Issue - 3 
Year of Publication : 2014
Authors : Raihanul Islam , Suvamoy Datta , Dayanidhi Sarkar


Raihanul Islam , Suvamoy Datta , Dayanidhi Sarkar. "In Vitro Detection of Uropathogenic Bacteria from UTI Infected Patients and Their Suitable Drugs Selection", International Journal of Biotech Trends and Technology (IJBTT), V4(3):11-27 Jul - Sep 2014. Published by Seventh Sense Research Group.


Urinary tract infections (UTI) are one of the most common infectious diseases diagnosed and management of urinary tract infections is complicated by the increasing prevalence of virulent antibiotic-resistant strains. The present study was designed to isolate the virulent organisms and their drug sensitivity status in a certain community. Total 100 urine samples were collected from patients with clinically diagnosed UTI attending both out-door and in-door patient from the prescription point. The isolates were identified by standard microbiological and biochemical process. From the total collected urine samples of the patients, 80% samples showed culture positive. Moreover, female patients were found more infected than male. Escherichia coli, Klebsiella spp & Staphylococcus spp were prevalent in all urine samples isolated from the diagnostic centers and subjected to characterizations namely cultural and biochemical identification, microscopic observation, identification of ESBL positive strain, antibiogram, and hemolytic assay study for the detection of virulence property. In this study 86% E. coli and 40% Klebsiella spp shown positive result for the presence of ? lactamase. E. coli, Klebsiella spp & Staphylococcus spp. showed multiple resistances of 6 to 8 antibiotics. E. coli and Klebsiella spp were 100% sensitive to imipenem and amikacin and compared to the other antibiotics tested and therefore these may be the drugs of choice for the treatment of UTIs.


[1] Orenstein R, Edward SW. "Urinary tract infections in adults." American family physician 1999:59.5: 1225-34.
[2] CDC (Centre for Disease Control). National Nosocomial Infection Study Report. Atlanta, Centre for Diseased Control 1979: pp. 2-14.
[3] Leigh D. Urinary Tract infections. In: Smith GR, Easma Charles SF, eds. Topley and Wilson’s principles of bacteriology, virology and immunity. Bacterial disease. 8th edition. Frome and London: Butler and Tanler Ltd. 1990:3: 197-214.
[4] Cattell WR. Infections of the kidney and Urinary Tract. Oxford University Press 1996: pp: 1-26.
[5] Foxman B. Epidemiology of urinary tract infection: Incidence, morbidity and ecomic costs. Dis. Mon. 2003: 49: 53-70.
[6] Gales AC, Sader HS, Jones RN. Urinary tract infection trends in Latin American hospitals. Diagn. Microbial. Infect. Dis. 2002: 44: 289-299.
[7] Thomas JG. Urinary Tract Infections. In: Diagnostic Microbiology 1995: pp: 950-969.
[8] Mudur G. Drug resistant cholera in India attributed to antibiotic misuse. BMJ. 2000:321:1368-9
[9] Steven AS. ‘‘Genitourinary tract” In: Current Medical Diagnosis and Treatment ELSEVIER New Youk. Amsterdam. Oxford. 1989: pp 595
[10] Jabbar HA, Moumena RA, Mosli HA, Khan AS, Warda A. Urinary tract Infection in pregnancy. Annal of Saudi Medicine. 1991:11:322-24
[11] Acharya VN. Urinary tract infection – a dangerous and unrecognized forerunner of systemic sepsis. j Postgrad Med. 1992: 38: 52-4.
[12] Mathai D, Jones RN, Pfaller MA. Epidemiology and frequency of resistance among pathogens causing urinary tract infection in 1,510 hospitalized patients: a report from the SENTY Antimicrobial Surveillance Program (North America) Diag Microbiol Infect Dis. 2001:40: 129-136.
[13] Karolwsky JA, Jones ME, Thornsberry C, Critchley I, Kelly L, Sahm D. Prevalence of antimicrobial resistance among pathogens isolated from female outpatients across the US in 1999. Int J Antimicrob Agents. 2001:18:121-127.
[14] Khan AU, Zaman MS. Multiple drug resistance pattern in Urinary Tract Infection patients in Aligarh. Biomedical Research. 2006: 17 (3): 179-181.
[15] Vasquez Y, Hand W L. Antibiotic Susceptibility Patterns of Community- Acquired Urinary Tract Infection Isolates from Female Patients on the US (Texas)- Mexico Border. The Journal of Applied Research. 2004: 4: No.2
[16] Prais D, Straussberg R, Avitzur Y, Nussinovitch M, Harel L, Amir J. Bacterial susceptibility to oral antibiotics in community acquired urinary tract infection. Arch Dis Child. 2003:88(3) : 215-218.
[17] Pelczar, Brian T, et al. "Identifying high-risk patients before head and neck oncologic surgery." Archives of Otolaryngology–Head & Neck Surgery 1993:119.8: 861-864.
[18] Bauer A W, Kirby W M M, Sherris J C T, Turck M. Antibiotic susceptibility testing by a standardized single disk method. American journal of clinical pathology 1966: 45(4): 493.
[19] Rahim ZS, Khan I, Chopra AK. "Biological characterization of Aeromonas spp. isolated from the environment." Epidemiology and infection 2004:132.04: 627-636.
[20] Valiquette L. Urinary tract infections in women. Canadian Journal of urology 2001: 8(1):6-12.
[21] Nahar SJ, Khanum H, Shimasaki K. Occurrence of Escherichia coli infection among the womwn of Dhaka city. ARPN Journal of Agricultural and Biological Science 2010: 5(6): 68-73.
[22] Leong CH. Urinary Tract Infection. Published by The Hong Kong College of General Practitioners 1978: 1(10):1-6.
[23] Hryniewicz K, Szezypa K, Sulikowska A, Jankowski K, Betlejewska K, Hryniewicz W. Antibiotic susceptibility of bacterial strains isolated from urinary tract infections in Poland. Journal of Antimicrobial Chemotherapy 2001:47: 773-780.
[24] Griebling TL. "Urinary tract infection in women." Urologic diseases in America 2007:7: 587-619.
[25] Kumar MS, Lakshmi V, Rajagopalan R. Occurrence of extended spectrum beta-lactamases among Enterobacteriacea spp. isolated at a tertiary care institute. Indian J. Med. Microbial 2006: 24: 208-11.
[26] Rizvi MF, Hasan Y, Memon AR, Abdullah M, Saleem S. Pattern of nosocomial infection in two intensive care units of a tertiary care hospital in Karachi. j Coll physicians Surg pak. 2007:17(3): 136-9.
[27] Sonavane A, Mathur M, Turbadkar D, Baradkar V. Antimicrobial Susceptibility Pattern in Urinary Bacterial Isolates. Bombay Hospital Journal, 2008:50:No. 2.
[28] Isaack H, Mbise RL, Hirji KF. Nosocomial bacterial infections among children with servere protein energy malnutrition. East Afr Med j. 1992:69(8): 433-6.
[29] Kahlmeter G. Prevalence and antimicrobial susceptibility of pathogens in uncomplicated cystitis in Europe. The ECO. SENS study. Int.J.Antimicrob. Agents. 2003:22:49-52.
[30] Gales AC, Jones RN, Gordon KA, Sader HS, Wilke WW, Beach ML, Pfaller MA, Doern GV. The SENTRY Study Group (Latin America). Activity and spectrum of 22 antimicrobial agents tested against urinary tract infection pathogens in hospitalized patients in Latin America: Report from the second year of the SENTRY antimicrobial surveillance program (1998). J. Antimicrob. Chemother 2000: 45: 295-303.

Urinary tract infections, drug resistance, Escherichia coli, Extended spectrum ?-lactamases (ESBL).