Successful Propagation of Pigeonpea (Cajanus cajan L.) through In Vitro Shoot Regeneration and Ex Vitro Rooting

Successful Propagation of Pigeonpea (Cajanus cajan L.) through In Vitro Shoot Regeneration and Ex Vitro Rooting

 
 		 International Journal of Biotech Trends and Technology (IJBTT)
 
© 2017 by IJBTT Journal
Volume - 7 Issue - 3                          
Year of Publication : 2017
Authors : Atul Dev, Ajinder Kaur
DOI :  10.14445/22490183/IJBTT-V23P601

Citation

Atul Dev, Ajinder Kaur "Successful Propagation of Pigeonpea (Cajanus cajan L.) through In Vitro Shoot Regeneration and Ex Vitro Rooting", International Journal of Biotech Trends and Technology (IJBTT), V7(3): 1-6 Jul - Sep 2017, Published by Seventh Sense Research Group.

Abstract

A reproducible in vitro shoot regeneration protocol was developed for pigeonpea variety ICPL 87 through proliferation of axillary buds from cotyledonary nodes. The seedlings were cultured on MS + Kin (2 mgL-1) + BAP (5 mgL-1) medium and de-capitated on 7th day of culturing, which resulted in the induction of multiple shoots from cotyledonary node region after 15 days of culturing. Miniature shoots were elongated on MS + adenine suphate (30 mgL-1) medium; the elongated shoots were rooted ex vitro using a rooting powder ROOTEXTM.

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Keywords
Callus, Regeneration, Direct regeneration, Multiple shoots.