Phytochemical analysis, Antioxidant and Antibacterial activity of Clitoriaternatea

 
 
International Journal of Biotech Trends and Technology (IJBTT)
 
© 2017 by IJBTT Journal
Volume - 7 Issue 1                          
Year of Publication : 2017
Authors : Najiya A, Alugoju Phaniendra, Krishnaswamy V K D3and Latha Periyasamy

Citation

Najiya A, Alugoju Phaniendra, Krishnaswamy V K D and Latha Periyasamy "Phytochemical analysis, Antioxidant and Antibacterial activity of Clitoriaternatea", International Journal of Biotech Trends and Technology (IJBTT), V7(1): 10-21 Jan to Mar 2017, Published by Seventh Sense Research Group.

Abstract

Medicinal plants are of great interest today because of their vast source of phytochemicals having potent health promoting activities. In the present study we investigated the phytochemical screening, antioxidant and antibacterial activity of root extract of Clitoriaternatea (butter fly pea). The results suggest that the root extract possess potent antioxidant activities such as reducing power assay, metal chelating assay, total antioxidant activity andantibacterial activity against pathogenic bacteria such asP.aerogenosa and B.subtilis. It is also showed that both aqueous and ethanol extracts possess flavonoid and phenolics. These results conclude that the root extract may be used as a potential antioxidant source.

References

1. Kampkötter, C. Timpel, R. F. Zurawski et al., “Increase of stress resistance and lifespan of Caenorhabditiselegans by quercetin,” Comparative Biochemistry and Physiology Part B, vol. 149, no. 2, pp. 314–323, 2008.
2. Bauer AW, Kirby WMM, Sherris JC, Turck M. Antibiotic susceptibility testing by standardized single disc method. Am. J. n. Pathol. 1966;36: 493–496.
3. Braude AI. Microbiology. London: W. B. Sauders Company; 1982.
4. Cook NC, Samman S: Flavonoids-chemistry, metabolism, cardioprotective effects, and dietary sources. J NutrBiochem 1996, 7:66-76.
5. Cook, B. G.; Pengelly, B. C.; Brown, S. D.; Donnelly, J. L.; Eagles, D. A.; Franco, M. A. ; Hanson, J.; Mullen, B. F.; Partridge, I. J.; Peters, M.; Schultze-Kraft, R., 2005. Tropical forages. CSIRO, DPI&F(Qld), CIAT and ILRI, Brisbane, Australia
6. Cook, B. G.; Pengelly, B. C.; Brown, S. D.; Donnelly, J. L.; Eagles, D. A.; Franco, M. A. ; Hanson, J.; Mullen, B. F.; Partridge, I. J.; Peters, M.; Schultze-Kraft, R., 2005. Tropical forages. CSIRO, DPI&F(Qld), CIAT and ILRI, Brisbane, Australia
7. Cowan MM. Plant products as antimicrobial agents. Clinical microbiology reviews 1999; 12(4): 564-582.
8. Cristea D, Bareau I &Vailarem G, Dyes Pigm, 57 (2003) 267
9. Devi BP, Boominathan R, Mandal SC.Anti-inflammatory, analgesic and antipyretic properties of Clitoriaternatea root. Division of Pharmacognosy, Faculty of Engineering and Technology, Jadavpur University, Calcutta 700 032, India. PMID: 12781804
10. Dnyaneshwar J Taur1* and Ravindra Y Patil2, Antihistaminic activity of Clitoriaternatea L. roots.Journal of Basic and Clinical Pharmacy-2011.
11. Dnyaneshwar J. Taura, Ravindra Y. Patilb, Evaluation of antiasthmatic activity of Clitoriaternatea L. roots.
12. Dwarakanath, C., The Fundamental Principles of Ayurveda. Part I – Introductory and Outlines of Nyaya–Vaisesika System of Natural Philosophy; Part II – Outlines of SamkhyaPatanjala System; Part III – Ayuskamiya and DravyadiVijnana (Including Rasabhediya), ChowkhambaKrishnadas Academy, Varanasi, India, 2003
13. Gao, J.J., Igalashi, K. and Nukina, M. (1999). Radical scavenging activity of phenylpropanoid glycosides in Caryopterisincana. Biosci. Biotech. Biochem. 63, 983-988.
14. Girish Kumar Gupta*, JagbirChahal, Manisha Bhatia., Clitoriaternatea (L.): Old and new aspects, JagbirChahal et al. / Journal of Pharmacy Research 2010, 3(11),2610-2614.
15. Gomez, S. M. ;Kalamani, A., 2003. Butterfly Pea (Clitoriaternatea): A nutritive multipurpose forage legume for the tropics - An overview. Pakistan J. Nutr., 2 (6): 374-379
16. Gomez, S. M. ;Kalamani, A., 2003. Butterfly Pea (Clitoriaternatea): A nutritive multipurpose forage legume for the tropics - An overview. Pakistan J. Nutr., 2 (6): 374-379
17. Haraguchi, H., Saito, T., Ishikawa, H., Date, H., Kataoka, S., Tamura, Y. and Mizutani, K.,1996. Antiperoxidative components in Thymus vulgaris. PlantaMedica, 62(3): 217-220.
18. Heaton PR, Reed CF, Mann SJ, et al: Role of dietary antioxidants to protect against DNA damage in adult dogs. J Nutr 2002;132:1720S-1724S.
19. Heim, K.E.; Tagliaferro, A.R.; Bobilya, D.J. Flavonoid antioxidants: Chemistry, metabolism and structure-activity relationships. J. Nutr. Biochem. 2002, 13, 572–584
20. Ito, N., Fukushima, S., Hagiwara, A., Shibata, M. and Ogiso, T. (1983). Carcinogenicity of butylatedhydroxyanisole in F344 rats. J. Nat Canc. Instit. 70: 343-347.
21. J.B morris. Characterization of butterfly pea (Clitoriaternatea L.) accessions for morphology, phenology, reproduction and potential nutraceutical, pharmaceutical trait utilization, Springer, Genet Resour Crop Evol (2009) 56:421-427 DOl 10. 1007/s 1 0722-008-9376-0
22. Katan MB, Hollman PC. Dietary flavonoids and cardiovascular disease. NutrMetabCardiovasc Dis. 1998;8:1–4.
23. Kiranmai s. rai*, k. dilipmurthy**, k. s. karanth*** and muddanna s. rao,clitoriaternatea (linn) root extract treatment during growth spurt period enhances learning and memory in rats,indian j physiolpharmacol 2001; 45 (3) : 305-313.
24. Kiranmai S. Rai, K. Dilip Murthy, Muddanna S. Rao, K. SudhakarKaranth,Altered dendritic arborization of amygdala neurons in young adult rats orally intubated with Clitoreaternatea aqueous root extract. Phytother Res. 2005, 19 (7) : 592-8.
25. KiranmaiS.Rai,Neurogenic Potential of ClitoriaternateaAqueous Root Extract–A Basis for Enhancing Learning and Memory, World Academy of Science, Engineering and Technology 46 2010.
26. Kitagawa S, Fujisawa H, Sakurai H. Scavenging effects of dihydric and polyhydric phenols on superoxide anion radicals, studied by electron spin resonance spectrometry. Chem Pharm Bull. 1992;40:304–7
27. Kumar R, Sharma RJ, Bairwa K, Roy RK, Kumar A. Pharmacological review on natural antidiarrhoel agents. Der PharmaChemica 2010; 2(2): 66-93
28. Lijy Jacob,*M.S. Latha, Anticancer activity of Clitoriaternatealinn. Against dalton’s lymphoma. International Journal of Pharmacognosy and Phytochemical Research 2012-13; 4(4); 107-112.
29. Malik J, Karan M, Vasisht K, Nootropic, anxiolytic and CNS-depressant studies on different plant sources of shankhpushpi.
30. Mathew S and Abraham T.E (2006). In Vitro Antioxidant Activity and Scavenging Effects of Cinnamomumverum Leaf Extract Assayed by Different Methodologies. Food Chemistry and Toxicology,Vol. 44, No. 2, pp. 198- 206. doi:10.1016/j.fct.2005.06.013
31. Melzig MF. Inhibition of adinosinedeaminase activity of aortic endothelial cells by selected flavonoids. Planta Med 1996;62:1-20
32. Ncube NS, Afolayan AJ, Okoh AI. Assessment techniques of antimicrobial properties of natural compounds of plant origin: current methods and future trends. African Journal of Biotechnology 2008; 7 (12): 1797-1806.
33. NeelmaniChauhan*, SaurabhRajvaidhya, B.K. Dubey.,Antiasthmatic effect of roots of clitoreaternatealinn.IJPSR (2012), Vol. 3, Issue 04.
34. Neeti N. Jain, C.C. Ohal, S.K. Shroff, R.H. Bhutada, R.S. Somani, V.S. Kasture, S.B. Kasture* , Clitoriaternatea and the CNS. Pharmacology, Biochemistry and Behavior 75 (2003) 529–536
35. Nguyen GiangKienTruc ; Sen Zhang; Nguyen NganThi Kim ; Nguyen Phuong QuocThuc ; Ming Sheau Chiu; Hardjojo, A. ; Tam, J. P., Discovery and characterization of novel cyclotides originated from chimeric precursors consisting of Albumin-1 chain and cyclotide domains in the Fabaceae family. J. Biol. Chem., 286 (27)2011: 24275–24287
36. Nguyen GiangKienTruc ; Sen Zhang; Nguyen NganThi Kim ; Nguyen Phuong QuocThuc ; Ming Sheau Chiu; Hardjojo, A. ; Tam, J. P., 2011. Discovery and characterization of novel cyclotides originated from chimeric precursors consisting of Albumin-1 chain a andcyclotide domains in the Fabaceae family. J. Biol. Chem., 286 (27): 24275–24287
37. Nino J, Narvaez DM, Mosquera OM and Correa YM. Antibacterial, antifungal and cytotoxic activities of eight Asteraceae and two Rubiaceae plants from Colombian biodiversity. Braz J Microbiol 37, (2006); 566-570.
38. Nisha Mathew & M. G. Anitha& T. S. L. Bala& S. M. Sivakumar& R. Narmadha& M. Kalyanasundaram. Larvicidal activity of Saracaindica, Nyctanthes arbor-tristis, and Clitoriaternatea extracts against three mosquito vector species. Springer, Parasitol Res (2009) 104:1017–1025. DOI 10.1007/s00436-008-1284-x
39. Nithianantham K, Shyamala M, Chen Y, et al. Hepatoprotective potential of Clitoriaternatea leaf extract against paracetamol induced damage in mice. Molecules. 2011;16(12):10134–10145. doi: 10.3390/molecules161210134 40. Ou B, Hampsch-Woodill M, Prior RL. Development and validation of an improved oxygen radical absorbance capacity assay using fluorescein as the fluorescent probe. J Nat Prod 63, (2001); 1035-1042
41. Parimaladevi B, Boominathan R, Mandal SC, Evaluation of antipyretic potential of Clitoriaternatea L. extract in rats. Division of Pharmacognosy and Phytochemistry, Department of Pharmaceutical Technology, Faculty of Engineering and Technology, Jadavpur University, Calcutta, India.
42. Patil AP, Patil VR. ClitoriaternateaLinn.: an overview. Int J Pharm Res. 2011;3:20–23.
43. Potapovich AI1, KostyukVA..Comparative study of antioxidant properties and cytoprotective activity of flavonoids Biochemistry (Mosc). 2003 May; 68(5):514-9, PMID: 12882632
44. Pradeep Kumar Gupta1 ,Dharmendra Kumar Varshney1 , Umesh Kumar2 and Bhavna. Screening of Antimicrobial Compound from Different Parts of Ziziphusziziphusxylopyrus international journal of pharmaceutical and chemical sciences, Vol. 2 (4) Oct-Dec 2013.
45. Pulok K. Mukherjeea,b,*, Venkatesan Kumara, N. Satheesh Kumara, MichealHeinrichb., The Ayurvedic medicine Clitoriaternatea—From traditional use to scienti?cassessment. Journal of Ethnopharmacology 120 (2008) 291–301
46. R. Prior and G. Cao (2000) Antioxidant phytochemicals infruits and vegetables, Diet and health implications. Hortic. Sci., 35, 588-592.
47. Rai KS, et al Clitoriaternatea root extract enhances acetylcholine content in rat hippocampus .Fitoterapia. (2002).
48. Ramkissoon JS, Mahomoodally MF, Ahmed N, Subratty AH. Antioxidant and anti-glycation activities correlates with phenolic composition of tropical medicinal herbs. Asian Pac J Trop Med. 2013;6:561–9. doi: 10.1016/S1995-7645(13)60097-8
49. Rani SA and Murty SU, Antifungal potential of flower head extract of Spilanthesacmella Linn. Afr J Biomed Res 9, (2006); 67-69.
50. Rice-Evans, C. (2004). Flavonoids and isoflavones: absorption, metabolism and bioactivity. Free Rad. Biol.Med. 36: 827-828.
51. Roggers YS, John LI, Mark LW. General Microbiology. 5th ed. Macmillan education Ltd London: 1990. 626-42.
52. SegenetKelemu *, César Cardona, Gustavo Segura, Antimicrobial and insecticidal protein isolated from seeds of Clitoriaternatea, a tropical forage legume Centro Internacional de Agricultura Tropical (CIAT), A.A. 6713 Cali, Colombia. / Plant Physiology and Biochemistry 42 (2004) 867–873
53. Shanthi P, Amudha P, Evaluation of the phytochemical constituents of Acmellacalva (dc.) r.k.jansen. Int J Pharm Biosci 1, (2010); B308 - B314.
54. Singleton, V. L.,& Rossi, J.A.Jr., Colorimetric of total phenolics with phosphomolebdic-phosphotungstic acid reagent. American journal of Enology and Viticulture, 16, (1965); 144-158.
55. Sivaprabha .J, Supriya. S., Sumathi, S., Padma, P.R., Nirmaladevi, R. and Radha. P. A study on the levels of nonenzymic antioxidants in the leaves and flowers of Clitoriaternatea. Department of Biochemistry, Biotechnology and Bioinformatics, Avinashilingam University for Women. Vol : No. XXVII (4) April, May, June – 2008
56. Soundrapandian C, Datta S, Sa B. Drug-eluting implants for osteomyelitis. Crit Rev Ther Drug-Carrier-Syst.2007;24:493545.,doi:10.,1615/CritRevTherDrugCarrierSyst.v24.i6.10.
57. Staples, 1992. ClitoriaternateaL.. Record from Proseabase. Mannetje, L.`t and Jones, R.M. (Editors). PROSEA (Plant Resources of South-East Asia) Foundation, Bogor, Indonesia
58. Staples, 1992. Clitoriaternatea L.. Record from Proseabase. Mannetje, L.`t and Jones, R.M. (Editors). PROSEA (Plant Resources of South-East Asia) Foundation, Bogor, Indonesia
59. Vidhyaramaswamy*, neethuvarghese, ancysimon, An investigation on cytotoxic and antioxidant properties of clitoriaternatea l. International Journal of Drug Discovery, ISSN: 0975–4423 & E-ISSN: 0975–914X, Vol. 3, Issue 1, 2011, pp-74-77.
60. Villanueva Avalos, J. F. ; Bonilla Cárdenas, J. A. ; Rubio Ceja, J. V. ; Bustamante Guerrero, J. de J., 2004. Agrotechnics and use ofClitoriaternatea in beef and milk production systems. TécnicaPecuaria en México, 42 (1)
61. Villanueva Avalos, J. F. ; Bonilla Cárdenas, J. A. ; Rubio Ceja, J. V. ; Bustamante Guerrero, J. de J., 2004. Agrotechnics and use ofClitoriaternatea in beef and milk production systems. TécnicaPecuaria en México, 42 (1)
62. Yen GC, Chen HY, Antioxidant activity of various tea extracts in relation to their antimutagenicity. J Agric Food Chem 43, (1995); 27-32.
63. Yildirim A, Mavi A, Oktay M, Kara AA, Algur OF, Bilaloglu V: Comparison of antioxidant and antimicrobial activities of Tilia(TiliaargenteaDesf Ex DC), Sage (Saviatriloba L.), and Black Tea (Camellia sinensis) extracts. J Agric Food Chem 2000,48(10):5030-5034.
64. Yildirim, A., Mavi, A., and Kara, A. .,Determination of antioxidant and antimicrobial activities of Rumexcrispus L. extracts. Journal of Agricultural and food chemistry, 49, (2001) ;4083-4089.
65. Zhang X, Xiao HB, Xue XY, Sun YG, Liang XM. Simultaneous characterization of isoflavonoids and astragalosides in two Astragalus species by high-performance liquid chromatography coupled with atmospheric pressure chemical ionization tandem mass spectrometry. J Sep Sci. 2007;30:2059–69. [PubMed]
66. Zu YG, Fu YJ, Liu W, Hou CL, Kong Y (2006) Simultaneous determination of four flavonoids in pigeonpeaCajanuscajan (L.) Millsp. leaves using RP-LC-DAD. Chromatographia 63(9–10):499–505, doi:10.1365/s10337-006-0784

Keywords
In vitro, Populus tremula, hybrid poplar, salinity.