On-Line Monitoring of Biofilm Forming Pseudomonas Sp on Stainless Steel Electrodes by Repetitive Cyclic Voltammetry

International Journal of Biotech Trends and Technology (IJBTT)
© 2016 by IJBTT Journal
Volume - 6 Issue - 4                          
Year of Publication : 2016
Authors : S. Muthukumaran, K.Jagadeesh, V.Srividhya
DOI :  10.14445/22490183/IJBTT-V19P602


S. Muthukumaran, K.Jagadeesh, V.Srividhya "On-Line Monitoring of Biofilm Forming Pseudomonas Sp on Stainless Steel Electrodes by Repetitive Cyclic Voltammetry", International Journal of Biotech Trends and Technology (IJBTT),V6(4): 6-9 Oct - Dec 2016, Published by Seventh Sense Research Group.


Biofouling monitors available presently do not detect biofilm formation directly. Some measure the reduction in heat transfer and pressure drop which can happen after considerable growth of biofilm formation typically greater than 30?m. By that time chlorine cannot penetrate these biofilms. There are some electrochemical techniques that detect corrosion initiation under the biofilms. This is a concern for the life and stability of biofilm probes. The main objective of the present study was to develop an electrochemical detector based on cyclic voltammetry on electrodes made of materials used in cooling water systems. Repetitive cyclic voltammetry is a technique that provides information on the redox reactions taking place at the metal electrolyte interface. Earlier work on biofilmed platinum electrodes has shown that area reduction on the surface due to biofilm formation suppresses the peak for oxidation of adsorbed hydrogen thereby indicating direct biofilm formation. In this study we used stainless steel electrodes with biofilms of a gram negative bacterium Pseudomonas sp. Studies with stainless steel electrodes exhibited very distinct responses between clean and biofilmed conditions providing several parameters for biofilm monitoring like lowering of peak height at the oxidation of adsorbed hydrogen peak, lower current density at the peakand lower charge density under 3h, 6h, 24h and 48h biofilms. Thus this preliminary study has confirmed the potential of cyclic voltammetry for online monitoring of early biofilm formation.


[1] R.A. Illsley, S.G.Roscoe, E.D. Jackson, T.J.Hughes, Biofouling 11 (1997) 191.
[2] W.G.Characklis, “Biofilm development and destruction” Electric power research institute. U.S .Report (1980) 902.1
[3] P.Cristiani, A.Mollica, G.Ventura, “On-line monitoring of biofilm” and T.R.O. by a new ENEL_S system, in: T.R. Bott (Ed), Understanding Heat exchanger fouling and its mitigation conference, 11-16 May, Castel Pascoli, Italy, Begell House, inc., New York, (1997), p. 323,329.
[4] M.W.Mittelman, L.L.Kohring, D.C.White, “Multipurpose laminar-flow adhesion cells for the study of bacterial colonization and biofilm formation” Biofouling 6 (1992) 39-51
[5] D.Pletcher, “The electrochemical consultancy” Hants U.K. A First Course in Electrode Processes (1991)

Biofilms, MIC (Microbial Influenced Corrosion), cyclic voltammetry, Stainless steel electrodes.