Citation :

Nargis Khan, Pratima Khare, Hemant Kumar Rawat, "Characterization and Identification of Alkali Stable Protease Produced by Citrococcous Paritis using Skimmed Milk as a Substrate," International Journal of Biotech Trends and Technology (IJBTT), vol. 15, no. 3, pp. 1-7, 2025. Crossref, https://doi.org/10.14445/22490183/IJBTT-V15I3P101

Abstract

Citrococcous paritis was isolated and identified as an alkali-stable protease using 16S rRNA sequencing analysis. After 48 h of incubation in the CPYA medium with 1% casein and 1% yeast extract (pH 10.0), an isolate of the detergent factory (DF-10) showed maximum protease activity of 18.10 U/ml when haemoglobin was used as substrate. The alkaline protease showed optimal activity at pH 10.0 and a temperature of 50°C. The newly isolated C. paritis strain was efficiently characterized for alkaline protease production and blood stain removal, and therefore can be used as a suitable candidate in the detergent and leather industry.

Keywords

Citrococcous paritis, Alkaline protease, Casein, Detergent, Alkali stable.

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