Penicillin G acylase producing bacteria isolated from forest soil

Penicillin G acylase producing bacteria isolated from forest soil

 
 		 International Journal of Biotech Trends and Technology (IJBTT)
 
© 2016 by IJBTT Journal
Volume - 6 Issue - 1                         
Year of Publication : 2016
Authors : Arjun. J K, Aneesh. B, Kavitha. T, Harikrishnan. K

Citation

Arjun. J K, Aneesh. B, Kavitha. T, Harikrishnan. K "Penicillin G acylase producing bacteria isolated from forest soil", International Journal of Biotech Trends and Technology (IJBTT), V6(1): 7-11 Jan - Mar 2016, Published by Seventh Sense Research Group.

Abstract

The development of resistance by microorganisms to penicillin G and other ? lactam antibiotics has prompted the search for new ? lactam antibiotics. Microorganisms are an important source of penicillin G acylase (PGA) (EC 3.5.1.11), which hydrolyses penicillins to 6-amino penicillanic acid (6-APA) and 7-amino des-acetoxy cephalosporanic acid (7-ADCA), the ? lactam antibiotic intermediates which are widely used by the pharmaceutical industries as the starting material for the manufacture of several semi synthetic antibiotics. In this study penicillin g acylase producing bacterial strains were isolated from the forest soil in Western Ghats. The isolated bacterial strains were screened for PGA production and the positive strain (RG_PGA 269) showing the highest activity was selected for further studies. The bacterial strain showed 2.22 U/ml enzyme activities. The presence of PGA gene in the isolate was confirmed by PCR using the gene specific primers. Molecular identification of the strain was done by 16 S rRNA gene amplification, sequencing and homology analysis of the sequence using NCBI BLAST. The Phylogenetic analysis of the strain was studied along with selected sequences from database and found that the isolate formed cluster with Bacillus megaterium.

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Keywords
PGA, 6-APA, ? lactam antibiotics, 16s rRNA gene, NCBI BLAST.