Effect of the sensitization to Dermatophagoïdes pteronyssinus on the inflammatory response and bronchoconstrictive of brown Norway rats

Effect of the sensitization to Dermatophagoïdes pteronyssinus on the inflammatory response and bronchoconstrictive of brown Norway rats

 
 		 International Journal of Biotech Trends and Technology (IJBTT)
 
© 2016 by IJBTT Journal
Volume - 6 Issue - 1                          
Year of Publication : 2016
Authors : Boutchi M, Roux E, Moumouni H, Maïzoumbou D.A, Alhousseini D

Citation

Boutchi M, Roux E, Moumouni H, Maïzoumbou D.A, Alhousseini D "Effect of the sensitization to Dermatophagoïdes pteronyssinus on the inflammatory response and bronchoconstrictive of brown Norway rats", International Journal of Biotech Trends and Technology (IJBTT), V6(1): 1-6 Jan - Mar 2016, Published by Seventh Sense Research Group.

Abstract

Asthma is a chronic inflammatory disease whose clinical consequences are airway obstruction. We sensitized rats brown Norwway with allergenic proteins from Dermatophagoides pteronyssinus (Der P1 and Der P2) for a better understanding of the pathophysiological mechanisms of this disease. Male brown Norway rats (300-400 g) were sensitized by 2 subcutaneous injections with proteins allergens (Der p1 and Der p2) (50 IR/ml) (Stallergenes AS, France) and Al2O3 at days 0 (D0) and 3 (D3), followed at D17 by intratracheal instillation of Der p. Control (C) rats (n=5) underwent the same protocol but with saline solution instead of Der p. Witness (W) rats (n=5) were not submitted to any treatment. At D24, enhanced expiratory pause (Penh), used as an index of airway resistance, was measured using a barometric plethysmograph for conscious animals. At D25, rats were killed, a bronchoalveolar lavage (BAL) was performed, and isometric contraction was measured on rings isolated from trachea (T), extrapulmonary (EPB) and intrapulmonary bronchi (IPB) using an organ bath system. Maximal contraction (Fmax) and LogEC50 were derived from cumulative concentration response curves to -8 to-3 LogM carbachol (CCh). The parameters measured are: airways resistance, inflammatory cells infiltration in BAL, allergenspecific response, and hyperresponsiveness and hypersensitivity of the airways to muscarinic agonists. In BAL fluid, cellular density was significantly higher in sensitized rats (334 ± 52 cells/?l) versus Control (217 ± 18 cells/?l) and versus Witness (165 ± 52 cells/?l) rats, as was the percentage of eosinophils (S: 8.75 %; C: 1.74 %; W: 0.7 %) and mast cells (S: 0.5 %; C: 0.2 %; W: 0 %). Allergen challenge increased Penh in sensitized rats, but not in Control and Witness rats. In vitro stimulation by Der p induced contraction of trachea, EPB and IPB rings isolated from S, but not C and W rats. In response to metacholine (MCh) challenge, MCh concentration (in LogM) inducing 300 % increase in Penh was significantly lower in sensitized rats (-4.36 ± 0.44) and Control (-3.92 ± 0.54) versus Witness rats (-2.86 ± 0.55). LogEC50 is significantly lower in sensitized rats (-6.22 ± 0.03; versus Control (-5.64 ± 0.02); and Witness rats (-5.23 ± 0.02). Sensitized rats showed (i) in vivo and ex vivo specific bronchoconstriction to allergen stimulation, (ii) in vivo hyperresponsiveness and ex vivo hyperreactivity and hypersensitivity to cholinergic stimulation, and (iii) increased proportion of eosinophils and mast cells in BAL fluid, indicating that such sensitized rats are a relevant model of asthma.

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Keywords
asthma, Dermatophagoïdes pteronyssinus, airways.