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International Journal of Biotech Trends and Technology (IJBTT) |  |
| © 2024 by IJBTT Journal | ||
| Volume - 14 Issue - 1 | ||
| Year of Publication : 2024 | ||
| Authors : Sabah Raheem Hamady, Ismail Jumah Abbass, Kadhim Fadhil Kadhim | ||
| DOI : 10.14445/22490183/IJBTT-V14I1P101 |
How to Cite?
"Detection of Biosynthesis of CuNPs using P. aeruginosa Isolated from Soil" International Journal of Biotech Trends and Technology vol. 14, no. 1, pp. 1-5, 2024. Crossref, https://doi.org/10.14445/22490183/IJBTT-V14I1P101
Abstract
The production of nanoparticles, while controlling the size and shape of the particles, has recently occupied much research in the field of nanotechnology. The biosynthesis of metal nanoparticles is of great importance due to their suitability for the environment. The use of microorganisms to produce nanoparticles has progressed over chemical and physical methods due to their effectiveness, low cost, and environmentally friendly nature. The present study focused on the synthesis of copper nanoparticles (CuNPs) in a simple, efficient, and rapid manner by the bacterium Pseudomonas aeruginosa. Copper is important in this process due to its interesting physical and chemical properties. It has good therapeutic potential, and when copper-sulphate-solution is added to the bacterial suspension devoid of bacterial cells, it is observed that the color of the reaction mixture changes to green, and this is evidence of the formation of nanoparticles. Different techniques were relied upon to characterize CuNPs by ultraviolet spectroscopy, with the highest value at 314. An FT-IR examination was conducted to determine the active aggregates. A SEM examination was also conducted, confirming that the size of copper nanoparticles reached 56.40 nm. This study aimed to produce nanoparticles. CuNPs using a Pseudomonas aeruginosa.
Keywords
Bacteria, CuNPs, UV-Spectroscopy, SEM.
References
[1] Naheed Ahmad, and Seema Sharma, “Green Synthesis of Silver Nanoparticles Using Extracts of Ananas Comosus,” Green and Sustainable Chemistry, vol. 2, no. 4, pp. 141-147, 2012.
[CrossRef] [Google Scholar] [Publisher Link]
[2] Inaam M. N. Alrubayae, and Kadhim F. Kadhim, “Determination of Oleaginous from Non-Oleaginous Fungi Using Enzymatic and Microscopic Techniques,” IOP Conference Series: Materials Science and Engineering, vol. 928, no. 6, pp. 1-10, 2020.
[CrossRef] [Google Scholar] [Publisher Link]
[3] Gadi Borkow et al., “Molecular Mechanisms of Enhanced Wound Healing by Copper Oxide‐Impregnated Dressings,” Wound Repair and Regeneration, vol. 18, no. 2, pp. 266-275, 2010.
[CrossRef] [Google Scholar] [Publisher Link]
[4] Gadi Borkow, Richard C. Zatcoff, and Jeffrey Gabbay, “Reducing the Risk of Skin Pathologies in Diabetics by using Copper Impregnated Socks,” Medical Hypotheses, vol. 73, no. 6, pp. 883-886, 2009.
[CrossRef] [Google Scholar] [Publisher Link]
[5] Geo. F. Brooks et al., Jawetz, Melnick and Adelberg’s Medical Microbiology, 25th ed., New York: McGraw-Hill Education, pp. 1-832, 2012.
[Google Scholar] [Publisher Link]
[6] Samuel Tertius Cowan, and Kenneth John Steel, Manual for the Identification of Medical Bacteria, Cambridge: University Press, London, pp. 1-217, 1965.
[Google Scholar] [Publisher Link]
[7] Jiale Huang et al., “Biosynthesis of Silver and Gold Nanoparticles by Novel Sundried Cinnamomum Camphora Leaf, Nanotechnology, vol. 18, no. 10, 2007.
[CrossRef] [Google Scholar] [Publisher Link]
[8] Akansha Treeza Joseph, Poonam Prakash, and S. S Narvi, “Phytofabrication and Characterization of Copper Nanoparticles using Allium Sativum and its Antibacterial Activity,” International Journal of Science, Engineering and Technology, vol. 4, no. 2, pp. 463-472, 2016.
[Google Scholar] [Publisher Link]
[9] Kadhim Fadhil Kadhim, and Inaam Mahmood Alrubayae, “Study of Lipase Production and Lipids Accumulation of Oleaginous Fungi Isolated from Oil-Rich Soil in Basrah, Scientific Journal of Medical Research, vol. 3, no. 12, pp. 123-127, 2019.
[Google Scholar] [Publisher Link]
[10] S. Nair Lakshmi, and Cato T. Laurencin, “Silver Nanoparticles: Synthesis and Therapeutic Applications,” Journal of Biomedical Nanotechnology, vol. 3, no. 4, pp. 301-316, 2007.
[CrossRef] [Google Scholar] [Publisher Link]
[11] Carlos Pecharromán et al., “Monodisperse and Corrosion‐Resistant Metallic Nanoparticles Embedded into Sepiolite Particles for Optical and Magnetic Applications,” Journal of the American Ceramic Society, vol. 89, no. 10, pp. 3043-3049, 2006.
[CrossRef] [Google Scholar] [Publisher Link]
[12] S. Shantkriti, and P. Rani, “Biological Synthesis of Copper Nanoparticles using Pseudomonas Fluorescens,” International Journal of Current Microbiology and Applied Sciences, vol. 3, no. 9, pp. 374-383, 2014.
[Google Scholar] [Publisher Link]
[13] Susheela Sharma et al., “Green Synthesis of Silver Nanoparticles and Their Antimicrobial Activity against Gram Positive and Gram Negative Bacteria,” International Journal of Biotechnology and Bioengineering Research, vol. 4, no. 4, pp. 341-346, 2013.
[Google Scholar] [Publisher Link]
[14] Ashwani Kumar Singh et al., “Biosynthesis of Gold and Silver Nanoparticles by Natural Precursor Clove and Their Functionalization with Amine Group,” Journal of Nanoparticle Research, vol. 12, pp. 1667-1675, 2010.
[CrossRef] [Google Scholar] [Publisher Link]
[15] Mradul Tiwari et al., “Biosynthesis of Copper Nanoparticles using Copper-Resistant Bacillus Cereus, A Soil Isolate,” Process Biochemistry, vol. 51, no. 10, pp. 1348-1356, 2016.
[CrossRef] [Google Scholar] [Publisher Link]
[16] Mradul Tiwari et al., “Biosynthesis and wound Healing Activity of Copper Nanoparticles,” IET Nanobiotechnology, vol. 8, no. 4, pp. 230-237, 2014.
[CrossRef] [Google Scholar] [Publisher Link]