The Biochemical Interplay between MCP1 and ATR
||International Journal of Biotech Trends and Technology (IJBTT)|
|© 2015 by IJBTT Journal|
|Year of Publication : 2015|
|Authors : Elsa Bronze-da-Rocha, Chii-Mei Lin, Mirit I. Aladjem|
Elsa Bronze-da-Rocha, Chii-Mei Lin, Mirit I. Aladjem "The Biochemical Interplay between MCP1 and ATR", International Journal of Biotech Trends and Technology (IJBTT),V12:1-6 September-October 2015. Published by Seventh Sense Research Group.
The maintenance of genome integrity requires and is regulated by two PIKKs [PI3K (phosphoinositide 3- kinase)-related kinases], the ATM (ataxia-telangiectasia mutated) and the ATR (ATM- and Rad3-related). ATR is the major coordinator of the response to DNA damage agents that interfere with progression of the replication fork and inhibit DNA synthesis. Since our previous work showed that MCP1 was associated with the early replication stages of DNA replication, we questioned whether MCP1 was required for the ATR pathway. Biochemical and immunological approaches revealed an association of MCP1 with ATR in undamaged and damaged cells. In damaged cells, the ATR-MCP1 interaction was primarily exhibited by the 31kDa form of MCP1. In undamaged cells, both 31kDa and 33kDa MCP1 isoforms interacted with ATR. The immunoprecipitation and immunofluorescence assays suggest that MCP1 may be involved in ATR pathway, namely by its association with ATR, under physiological conditions and after DNA damage.
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ATR, MCP1, DNA damage, cell cycle.