IJCOT-book-cover International Journal of Biotech Trends and Technology  (IJBTT)          
© 2022 by IJBTT Journal
Volume - 12 Issue - 3
Year of Publication : 2022
Authors : Bashir Ahmad Aseem, Mohammad Arif Ahsas
DOI :   10.14445/22490183/IJBTT-V12I3P601

How to Cite?

Bashir Ahmad Aseem, Mohammad Arif Ahsas. "Biosynthesis, Characterization and Antibacterial Properties of ZnO Nanoparticles of Cold Tolerant Microbacterium sp." International Journal of Biotech Trends and Technology  vol. 12, no. 3, pp. 1-7, 2022. Crossref, https://doi.org/10.14445/22490183/IJBTT-V12I3P601 


Nanotechnology has attracted great interest recently due to its unexpected impact on many areas of science and life, especially in biology and biomedicine. Nanoparticles, due to their unique physical, chemical, optical, electronical, and magnetic properties, have increased interest in their synthesis. They have been synthesized by various physical and chemical processes, but most of these methods require a vast amount of energy or cannot avoid the formation of toxic byproducts in their synthesis process. Therefore, there is an urgent need to develop green processes for nanoparticle synthesis. Such as the biological synthesis of nanoparticles by bacteria has been considered. In this study, zinc oxide nanoparticles were biosynthesis by using microbial culture supernatant from the cold-tolerant strain of Microbacterium sp. OSNP13. The Biosynthesized nanoparticles were characterized by UV-Vis, DLS and XRD analysis. The results indicated that the average size of zinc oxide nanoparticles was 59.16 nm. The antibacterial activity of the produced nanoparticles was then evaluated. The MIC of zinc oxide nanoparticles for E. coli and S. aureus was also calculated to be 500 μg/ml. The zinc oxide nanoparticles produced in this study have shown good antimicrobial properties and can be suitable candidates for use as antimicrobial agents.


Antibacterial activity, Cold-tolerant microbacterium sp, Minimum bactericidal concentration, Minimum inhibitory concentration, Zinc oxide nanoparticles.


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