Diversity Analysis and Polymorphism Through RAPD Markers in Eucalyptus Tereticornis Sm
||International Journal of Biotech Trends and Technology (IJBTT)|
|© 2021 by IJBTT Journal|
|Volume - 11 Issue - 3
|Year of Publication : 2021|
|Authors : Shivani Dobhal, Ashok Kumar|
|DOI : 10.14445/22490183/IJBTT-V11I3P604|
MLA Style:Shivani Dobhal, Ashok Kumar "Diversity Analysis and Polymorphism Through RAPD Markers in Eucalyptus Tereticornis Sm" International Journal of Biotech Trends and Technology 11.3 (2021): 23-30.
APA Style:Shivani Dobhal, Ashok Kumar(2021). Diversity Analysis and Polymorphism Through RAPD Markers in Eucalyptus Tereticornis Sm. International Journal of Biotech Trends and Technology, 11(3), 23-30.
The genetic diversity of 25 genotypes of Eucalyptus tereticornis Sm. was analyzed using randomly amplified polymorphic DNA (RAPD) with 10 decamer primers. The number of scorable bands for each primer varied from 6(OPA-2) to 14 (M-131), with an average of 10 bands per primer. A total of 96 distinct DNA fragments (bands) were amplified, of which 80 were polymorphic, with172 to 1353 base pairs. The number of amplified bands per genotype varied from 5 to 13 and percentage polymorphism, from 73% to 93% with an average of 83.32%. The mean polymorphic information content (PIC) of RAPD primers was 0.34 and ranged from 0.19 to 0.44. Although genotypes originating from a single provenance tended to fall into the same cluster, those from the same location were dispersed across different clusters.
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Eucalyptus tereticornis, RAPD, genetic diversity, clustering