The Phylogenetic relationship study of Maturase K and Ribulose 1,5 bisphosphate carboxylase/oxygenase large subunit – A DNA barcoding marker region of Medicinal plant Beetroot (Beta vulgaris) from the region of Gujarat (INDIA)
 |
International Journal of Biotech Trends and Technology (IJBTT) |  |
| © 2021 by IJBTT Journal | ||
| Volume - 11 Issue - 3 |
||
| Year of Publication : 2021 | ||
| Authors : Shubham S Bumb, Dr. Sanjay Lal, Dr. Sandeep Chovatiya | ||
| DOI : 10.14445/22490183/IJBTT-V11I3P603 |
Citation
MLA Style:Shubham S Bumb, Dr. Sanjay Lal, Dr. Sandeep Chovatiya "The Phylogenetic relationship study of Maturase K and Ribulose 1,5 bisphosphate carboxylase/oxygenase large subunit – A DNA barcoding marker region of Medicinal plant Beetroot (Beta vulgaris) from the region of Gujarat (INDIA)" International Journal of Biotech Trends and Technology 11.3 (2021): 18-22.
APA Style:Shubham S Bumb, Dr. Sanjay Lal, Dr. Sandeep Chovatiya(2021). The Phylogenetic relationship study of Maturase K and Ribulose 1,5 bisphosphate carboxylase/oxygenase large subunit – A DNA barcoding marker region of Medicinal plant Beetroot (Beta vulgaris) from the region of Gujarat (INDIA). International Journal of Biotech Trends and Technology, 11(3), 18-22.
Abstract
Introduction: Beetroot, scientifically called Beta vulgaris, is one in every of the accepted plants belonging to the Chenopodiaceae family. It is associate with an erect annual herb with stalk rootstocks. It makes a superb dietary supplement being not solely made in minerals, nutrients, and vitamins; however, it conjointly has distinctive phytoconstituents that have many medicative properties. Several elements of this plant area unit are utilized in a meditative system like an anti-oxidant, anti-depressant, anti-microbial, anti-fungal, medicine, diuretic, medicinal drug, and carminative.
Aim of the study: To identify the medicinal plant using plant core barcode (matK and rbcL).
Materials and methods: A sample of the beetroot was collected from the main market of Anand city, Gujarat (INDIA). DNA samples were extracted, and it was amplified using matK and rbcL primer. The amplified product was sequenced at Eurofins Sequencing Lab, Bangalore, India. The sequence was edited manually using Chromatogram explorer. Species identification established by NCBI BLAST and constructing a phylogenetic tree using CLC main workbench.
Results: PCR amplification results gave 100 % success for both the loci and thus confirmed the amplification. BLAST analysis also confirmed the similarity to genus Beta, and phylogenetic tree analysis showed a best close match to identify the plant species.
Conclusion: DNA Barcoding is a reliable tool for species identification. Our result shows that the matK and rbcL proved efficient in identifying the plant species.
References
[1] Techen, N. et al., DNA barcoding of medicinal plant material for identification,” Current Opinion in Biotechnology, 25 (2014) 103–110. doi: 10.1016/j.copbio.2013.09.010.
[2] Hebert PDN, Ratnasingham S, de Waard JR. Barcoding animal life: cytochrome c oxidase subunit 1 divergence among closely related species. Proc R Soc Lond B Biol Sci 270 Barcoding animal life: cytochrome c oxidase subunit 1 divergences among closely. Proc Biol Sci. 7(270) (2003) Suppl 1:S96-9
[3] Kress, W.J. New Technologies for Taxonomy. Nat. Hist.Mini-barcodes, D.N. a., DNA Barcodes. 858 (2012) 339–353.
[4] Chase MW, Salamin N, Wilkinson M, et al., Land plants and DNA barcodes: short term and long-term goals. Phil Trans Lond B 360 (2005) 1889–1895
[5] Newmaster SG, Fazekas AJ, Ragupathy S ., DNA barcoding in the land plants: an evaluation of rbcL in a multigene tiered approach. Can J Bot 84 (2006) 335–341
[6] Vere, N. de et al., Dna barcoding for plants, Methods in Molecular Biology, 1245 (2015) 101–118. doi: 10.1007/978-1-4939-1966-6_8.
[7] Chen, S. et al., Validation of the ITS2 region as a novel DNA barcode for identifying medicinal plant species, PLoS ONE, 5(1) (2010) 1–8. doi: 10.1371/journal.pone.0008613.
[8] Hart, B. L., The evolution of herbal medicine: Behavioural perspectives, Animal Behaviour, 70(5) (2005) 975–989. doi: 10.1016/j.anbehav.2005.03.005.
[9] Sucher, N. J., and Carles, M. C., Genome-based approaches to the authentication of medicinal plants, Planta Medica, 74(6) (2008) 603–623. doi: 10.1055/s-2008-1074517.
[10] IEA., Chapter 1 ??? ?? Chapter 1 ???, An Automated Irrigation System Using Arduino Microcontroller, 1908 (2011) 2–6.
[11] Kumar, Y., Beetroot: A Super Food, Internal Journal of Engineering Studies and Technical Approach, 01(3) (2015) 1–7.
[12] Jasmitha, S., Shenoy, A. and Hegde, K., Review Article A REVIEW ON Beta vulgaris (BEETROOT), International Journal of Pharma And Chemical Research, 4(2) (2018) 136–140.
[13] Vences, M. et al., DNA Barcodes Amphibians and reptiles, DNA Barcodes: Methods and Protocols, 858 (2008) (2018). doi: 10.1007/978-1-61779-591-6.
[14] Al-Qurainy, F. et al., Selection of DNA barcoding loci and phylogenetic study of a medicinal and endemic plant, Plectranthus asirensis J.R.I. wood from Saudi Arabia, Genetics and Molecular Research, 13(3) (2014) 6184–6190. doi: 10.4238/2014.August.7.31.
[15] Kang, Y. et al., DNA barcoding analysis and phylogenetic relationships of tree species in tropical cloud forests, Scientific Reports, 7(1) (2017) 1–9. doi: 10.1038/s41598-017-13057-0.
[16] Kaur, S., DNA Barcoding and its Applications, Biotechnology in Horticulture: Methods and Applications, 3 (2013) 414.
[17] Keele, J. et al., Identification of Unknown Organisms by DNA Barcoding: A Molecular Method for Species Classification, Trends in Genetics, 23(4) (2014) 167–172. doi: Research and Development Office, Final report 2014-01 (0045).
[18] Letchuman, S., Short Introduction of DNA Barcoding, International Journal of Research, 5(4) (2018) 673–686.
[19] McGinnis, S. and Madden, T. L., BLAST: At the core of a powerful and diverse set of sequence analysis tools, Nucleic Acids Research, 32(WEB SERVER ISS.) (2004) 20–25. doi: 10.1093/nar/gkh435.
[20] Horner, D. S. et al. A unified index of sequence quality and contig overlap for DNA barcoding. Pakistan Journal of Botany 7 (2015) 3190–3194.
[21] Anvarkhah, S., Khajeh-Hosseini, M., and Rashed, M.H., Identification of three species of genus Allium using DNA barcoding. 1. international Journal of Agriculture and Crop Sciences 5 (11) (2013) 1195
[22] Porebski S, et al., Modification of a CTAB DNA extraction protocol for plants containing high polysaccharide and polyphenol components. Plant Biol. Reptr., 15 (1997) 8-15.
Keywords
DNA Barcoding, Species identification, rbcL, matK, Phylogenetic tree, BLAST, NCBI