IJBTT

Media Optimization And Saccharification of Lignocellulosic Waste By Cellulolytic Bacteria Isolated From Gut of A Termite

	International Journal of Biotech Trends and Technology (IJBTT)
	© 2021 by IJBTT Journal
	Volume - 11 Issue - 2
	Year of Publication : 2021
	Authors : Yash Varma, Sandeep Chovatiya, Dipika Patel, Snehal Ingle, Nisha Daxini, Sanjay Lal
	DOI :  10.14445/22490183/IJBTT-V11I2P602

Citation

MLA Style:Yash Varma, Sandeep Chovatiya, Dipika Patel, Snehal Ingle, Nisha Daxini, Sanjay Lal  "Media Optimization And Saccharification of Lignocellulosic Waste By Cellulolytic Bacteria Isolated From Gut of A Termite" International Journal of Biotech Trends and Technology 11.2 (2021): 8-14.

APA Style:Yash Varma, Sandeep Chovatiya, Dipika Patel, Snehal Ingle, Nisha Daxini, Sanjay Lal 2021). Media Optimization And Saccharification of Lignocellulosic Waste By Cellulolytic Bacteria Isolated From Gut of A Termite. International Journal of Biotech Trends and Technology, 11(2), 8-14.

Abstract

Cellulase creation from bacteria can be a favourable position as the enzyme generation rate is typically higher because of the higher bacterial development rate when contrasted with fungi. Screening of bacteria, advancement of fermentation conditions and choice of substrates are imperative for the fruitful creation of cellulase. This study was conducted from isolation of cellulolytic bacteria from Damp wood termite. They were similar like Citrobacter, Enterobacter and Cellulomonas and using media optimization method for highest production of cellulase and using this cellulase for saccharification of lignocellulosic waste. The highest cellulase producing ability of isolate from this study was found to be 51.9 IU/ml with 48 hours of incubation and as carboxymethyl cellulose as substrate. Treated and untreated corncob was subjected to saccharification. Highest saccharification was observed with 15gm of substrate treated with 3% NaOH at 50ºC for 30min.

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Keywords
Termites, Cellulolytic Bacteria, Gram Staining, Media Optimization, Placket – Burman Method, Saccharification, Corn cob