Quantitative estimation of Embelin from Embelia tsjeriam-Cottam A.DC. and in vitro multiplication for production of quality planting stock
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International Journal of Biotech Trends and Technology (IJBTT) |  |
| © 2021 by IJBTT Journal | ||
| Volume - 11 Issue - 1 |
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| Year of Publication : 2021 | ||
| Authors : Shailendra Kumar Tiwari, M.P. Goswami, Pankaj Saini | ||
| DOI : 10.14445/22490183/IJBTT-V11I1P601 |
Citation
MLA Style:Shailendra Kumar Tiwari, M.P. Goswami, Pankaj Saini"Quantitative estimation of Embelin from Embelia tsjeriam-Cottam A.DC. and in vitro multiplication for production of quality planting stock" International Journal of Biotech Trends and Technology 11.1 (2021): 1-6.
APA Style:Shailendra Kumar Tiwari, M.P. Goswami, Pankaj Saini(2021).Quantitative estimation of Embelin from Embelia tsjeriam-Cottam A.DC. and in vitro multiplication for production of quality planting stock. International Journal of Biotech Trends and Technology, 11(1), 1-6.
Abstract
The genus Embelia tsjeriam-Cottam A.DC. belongs to the family Myrsinaceae. It is a shrub in nature. Globally the species is recorded from India, Myanmar, and Sri Lanka. It is found in India found in Maharashtra, Karnataka, Kerala, Tamil Nadu, Chhattisgarh, and Andhra Pradesh. Embelin, a benzoquinone, and gallic acid 2, polyphenol compounds are reported in species as antioxidant and anticancer properties. Presently the species is under the rare category. Therefore, it is an urgent need to develop an appropriate propagation technology for its conservation and multiplication. This paper highlights the quantitative estimation of bioactive compound Embelin through HPLC for the identification of quality germplasm. The identified quality planting material was subjected to their in vitro multiplication.
Germplasm of Borivali forest area, Maharashtra showed optimum 4.88% Embelin. Optimum in vitro morphogenetic response in terms of shoot multiplication was achieved when the MS culture medium was supplemented with BAP 3.0mg/lt + IAA 1.0mg/lt. Average 22 green and healthy shoots with 6.16cm length were induced. The in vitro regenerated shoots were shifted forex vitro rooting. IBA 100ppm solution showed an excellent rooting response. After rooting, the plants were acclimatized and hardened in a greenhouse with more than 74% plant recoveries.
References
[1] Babu Ganesan, Chemical constituents of Embelia basaal (Roem. & Schult.) DC. Journal of Pharmacy Research 2(10) (2009), 1575-1578.
[2] Basak UC & Mohapatra M, Quantitative reckoning of embelin from fruits of Embelia tsjeriam-Cottam using water bath process as an alternate method of extraction, Indian Journal of Pharmaceutical and Biological Research 3(03) (2015).
[3] Malik Sonia, Sharma Madhu & Ahuja Paramvir Singh, An efficient and economic method for in vitro propagation of Arnebia euchroma using the liquid culture system American Journal of Biotechnology Medical Research DOI: 10.5455/ajbmr., 20160501100040, (2016).
[4] Murashige Toshio & Skoog Folke, A Revised Medium for Rapid Growth and Bio Assays with Tobacco Tissue Cultures, Physiologia Plantarum, 15, (1962).
[5] Rajan Dilna RA, Raghur V, Hrideek TK, & Muralidharan EM, Micropropagation of Embelia tsjeriam-Cottam - an endangered medicinal plant of western ghats, Proceedings of the 8th National Seminar on Medicinal plants. KFRI, Kerala (2013).
[6] Tiwari SK, Krishnamurthy G, Goswami MP, Pandey Amit & Singhal PK, In Vitro Propagation of Litsea glutinosa (Lour) C.B. Robinson An Endangered Medicinal Tree in Madhya Pradesh, India. International Journal of Current Research in Biosciences and Plant Biology, 2( 12)(2015), 75-79.
[7] Subramaniam, Sudhakar Raja, Kuzhiumparambil, Unnikrishnan, Ravindran, PN & Balachandran, I. Determination of embelin Embelia Ribes and Embelia tsjeriam-Cottam by HPLC. Indian J. Pharm. Sci,. 67(2005), 734-736.
[8] Tiwari SK, Pandey Amit, Mishra Shweta & Pandey Rashi Quantitative determination of active alkaloid in Withania sominifera through high-performance liquid chromatography the production of quality planting stock. Journal of Tropical Forestry 28(3)(2012), 47-51.
Keywords
MS, bioactive compound, Embelin, in vitro, nodal, explants.