IJBTT

In vitro Propagation of Mentha pulegium L. and Testing, The Antifungal Activity of Stem Nodes Callus and Shoots Extracts Against Acremonium strictum L

	International Journal of Biotech Trends and Technology (IJBTT)
	© 2020 by IJBTT Journal
	Volume - 10 Issue - 3
	Year of Publication : 2020
	Authors : Reem Ebraheem, Daniel Al-awad, Maysa Yaziji
	DOI :  10.14445/22490183/IJBTT-V10I3P603

Citation

MLA Style:Reem Ebraheem, Daniel Al-awad, Maysa Yaziji"In vitro Propagation of Mentha pulegium L. and Testing, The Antifungal Activity of Stem Nodes Callus and Shoots Extracts Against Acremonium strictum L" International Journal of Biotech Trends and Technology 10.3 (2020): 14-19.

APA Style:Reem Ebraheem, Daniel Al-awad, Maysa Yaziji(2020). In vitro Propagation of Mentha pulegium L. and Testing, The Antifungal Activity of Stem Nodes Callus and Shoots Extracts Against Acremonium strictum L. International Journal of Biotech Trends and Technology, 10(3), 14-19.

Abstract

This research aims to propagate buds of Mentha pulegium and forming callus, then determine the antifungal activity of ethanol, methanol for both stem node callus extract, and shoots extract M.pulegium against Acremonium strictum. The stem nodes segments of M. pulegium were cut into 0.5-1 cm in length and cultured on Murashige and Skoog (MS) solid medium supplemented with 3% sucrose, and different concentration of benzyl amino purine (BAP) was added either alone to propagate buds or in combination with 2,4-Dichlorophenoxyacetic acid (2;4-D) to form a callus. The MS was gelled with 8% agar. Antifungal activity was performed in Petri dishes (poisoned food). The maximum number of callus (98.33±1.96%) was obtained on MS medium supplemented with 0.5mg/l BAP in combination with 1mg/l 2,4-D, and the maximum number of shoots (20±0.88) was obtained on MS medium supplemented with 1mg/l of BAP. The results showed a higher efficiency of ethanol extracts than those of methanol. Ethanol and methanol extracts of callus were generally better than shoots extracts. MIC ( Minimum Inhibition Concentration) value of the callus extract was 0.02 g / ml when using ethanol or methanol as an organic solvent, but the MIC value of the shoots extract was 0.03g/ml when using ethanol or methanol as an organic solvent.

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Keywords
Mentha pulegium, shoot, callus, Acremonium strictum, Antifungal activity.