IJBTT

Molecular Diversity Analysis of Different Hybrid Rice (Oryza sativa L.) Varieties through RAPD and SSR Markers

	International Journal of Biotech Trends and Technology (IJBTT)
	© 2020 by IJBTT Journal
	Volume - 10 Issue - 3
	Year of Publication : 2020
	Authors : Absana Islam, Shamim Ara Sumi, Abdul Wahab, M. E. Hoque
	DOI :  10.14445/22490183/IJBTT-V10I3P602

Citation

MLA Style:Absana Islam, Shamim Ara Sumi, Abdul Wahab, M. E. Hoque"Molecular Diversity Analysis of Different Hybrid Rice (Oryza sativa L.) Varieties through RAPD and SSR Markers" International Journal of Biotech Trends and Technology 10.3 (2020): 6-13.

APA Style:Absana Islam, Shamim Ara Sumi, Abdul Wahab, M. E. Hoque(2020). Molecular Diversity Analysis of Different Hybrid Rice (Oryza sativa L.) Varieties through RAPD and SSR Markers. International Journal of Biotech Trends and Technology, 10(3), 6-13.

Abstract

Rice (Oryza sativa L.) is one of the principal cereal crops around the globe. A study was conducted to assess molecular diversity of four rice hybrid varieties released from a private seed company using PCR-based Random Amplified Polymorphic DNA (RAPD) and Single Sequence Repeat (SSR) markers. A total of 20 RAPD primers and 4 SSR primers were used to determine polymorphism among the rice hybrids. Some total 76 DNA bands were amplified through RAPD primers. Among them 24 were polymorphic bands. The rate of polymorphism was obtained about 30.20%. Genetic diversity ranged from 0.083 to 0.438 and the frequency of major allele ranged from 0.625 to 0.917. The PIC value ranged from 0.063 to 0.340 with the average value 0.150. The PIC value indicated that the studied rice hybrids had low molecular diversity. The dendrogram indicating the relative genetic similarity of the rice hybrids was constructed which followed three major clusters (A, B and C) among the studied material. Two SSR primers showed polymorphic bands which can be used as an evidence of variety protection data. These experimental findings can be used for the protection of hybrids in commercial purpose and in managing rice genetic resource in Bangladesh.

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Keywords
Molecular Diversity, RAPD and SSR Markers, Polymorphism, Rice hybrid.