Production of Protein-Rich Biomass by the Consortium of Microscopic Fungi - Chaetomium cellulotycum A 43 and Sporotrichum pulverulentum A 32
Citation
MLA Style:Izolda Khokhashvili, Lali Kutateladze, Nino Zakariashvili, Tamar Urushadze,Maya Jobava , Rusudan Khvedelidze, Tamar Burduli, NinoTsiklauri , Tinatin.Aleksidze"Production of Protein-Rich Biomass by the Consortium of Microscopic Fungi - Chaetomium cellulotycum A 43 and Sporotrichum pulverulentum A 32" International Journal of Biotech Trends and Technology 10.1 (2020): 50-55.
APA Style:Izolda Khokhashvili, Lali Kutateladze, Nino Zakariashvili, Tamar Urushadze,Maya Jobava , Rusudan Khvedelidze, Tamar Burduli, NinoTsiklauri , Tinatin.Aleksidze. Production of Protein-Rich Biomass by the Consortium of Microscopic Fungi - Chaetomium cellulotycum A 43 and Sporotrichum pulverulentum A 32 International Journal of Biotech Trends and Technology, 10(1), 50-55.
Abstract
Nonpathogenic and nontoxic strains of microscopic fungi, from the collection of the Institute of Biochemistry and Biotechnology of the Agricultural University of Georgia have been screened for the selection of proteins active producers under the conditions of solid phase fermentation of the agricultural and food industry waste. Two active producers of proteins have been revealed: C. cellulotycum A 43 and S. pulverulentum A 32, as well as the perspective substrate for the bioconvertion – tomato-cake was selected. The optimal conditions for cultivation and composition of the nutritional medium for Ch. cellulotycum A 43 and Sp.pulverulentum A32 were established. Biomasses with 2.3-2.8 times higher content of pure protein, compared with control have been obtained on the base of optimization of the cultivation conditions and nutritional medium composition. The united cultivation of experimental strains was performed for the improvement of bioconversion degree. The synergetic effect of the combined cultivation of fungi on the tomato-cake has been demonstrated. The combined cultivation of C. cellulotycum A 43 and S. pulverulentum A 32 under the optimal conditions resulted in the production of sugar- (20%) and protein-rich (16.5%), easy-digestible, nontoxic and nonpathogenic biomass, which may be used as protein- and other bioactive substances-rich food additive.
References
[1] Oliveira, M.D., Feddern, V., Kupski, L., Cipolatti, E.P., Badiale-Furlong, E., & Souza-Soares, L.A. Changes in lipid, fatty acids and phospholipids composition of whole rice bran after solid- state fungal fermentation. Bioresource Technology, 2011; 102(17), 8335–8338
[2] Nigam P. S. nee’ , Pandey A. Solid-State Fermentation Technology for Bioconversion of Biomass and Agricultural Residues, Biotechnology for Agro - Industrial Residues Utilisation 2009; 197-221
[3] Tesfaye A. Method of Standardization and Determination of Protein Extraction Using Orange Wastes by Aspergillus niger Isolate in Solid State Fermentation. The manuscript is submitted to Ethiopian Journal of Biological Sciences 2014; 13(2)
[4] Kupski, L., Cipolatti, E., Rocha, M., Oliveira, M.D., Souza-Soares, L.D.,& Badiale Furlong, E. Solid-state fermentation for the enrichment and extraction of proteins and antioxidant s, Int J Cur Res Rev; 2017; 9 ( 21) compounds in rice bran by Rhizopus oryzae. Brazilian Archives of Biology and Technology, 2012; 5(6), 937–942.;
[5] Schmidt, C.G., Goncalves, L.M., Prietto, L., Hackbart, H.S., & Furlong, E.B. Antioxidant activity and enzyme inhibition of phenolic acids from fermented rice bran with fungus Rhizopus oryzae. Food Chemistry, 2014; 146, 371–377.
[6] Das, N., Mukherjee, M.,. Cultivation of Pleurotus ostreatus on weed plants. Biores. Technol., 2007; 98(14): 2723–2726
[7] Nasseri A.T. Rasoul-Amini S., Morowvat and Y.Ghasemi Single Cell Protein: Production and Process American Jurnal of Food Technology, 2011; 6(2):103-116
[8] Aggelopoulos, T.,. Katsieris K, Bekatorou A., Pandey A., Banat I.M and. Koutinas A.A. Solid state fermentation of food waste
[9] mixtures for single cell protein, aroma volatiles and fat production. Food chemistry. 2014; 145:710-716
[10] Jaganmohan, P., Purushottam B.and Prasad S.V.. Production of SCP with Aspergillus terrus using Solid State fermentation. Eur. J. Biol. Sci. 2013; 5(2):38-45
[11] Oshoma, Ge; Eguakun-Owe So, Conversion of Food waste to Single Cell Protein using Aspergillus Niger, J. Appl. Sci. Environ. Manage. 2018; 22 (3) 350 –355.
[12] FAO, Food and Agriculture Organization of the United Nations. Amino acid content of foods.Rome; 1970; 24.
[13] Ghose,T. K.; Measurement of cellulase activities. Pure andapplied chemistry 1987; 59(2); 257—268,
[14] Miller, G.L., Use of dinitrosalicylic acid reagent for determination of reducing sugar. Anal. Chem., 1959; 31, 426
[15] Tien M , Kirk T.K. Lignin-degrading enzyme from Phanerochaete chrysosporium: Purification, characterization, and catalytic properties of a unique H2O2- requiring oxygenase. Proc Natl Acad Sci US A. 1984; 81(8): 2280–2284.
[16] Updegraff D.M. Semimicro determination of Cellulose in Biological materials. – Analit . Biochem., 1969, 32( 2), 420-424
[17] Augustine O., Ayeni, Opeyemi, A., Adeeyo, Oyinlola M., Oresegun Temitayo, E. Oladimeji. Compositional analysis of lignocellulosic materials: Evaluation of an economically viable Appl method suitable for woody and non-woody biomass, American Journal of Engineering Research (AJER); 2015 4(4), 14-19.
[18] AOAC – Association of Official Analytical Chemists. Official methods of analysis (17th ed)
[19] Perinbam Kantharaj, Bharath Boobalan, Seeni Sooriamuthu, Ravikumar Mani, Lignocellulose Degrading Enzymes from Fungi and Their Industr
Keywords
Microscopic fungi; Solid state fermentation; Agricultural and food industry waste; Bioconversion; Protein-rich biomass;