In Vitro Micro Tuber Formation in Potato (Solanum tuberosum L.): is there any Relation between Methyl Jasmonate, Sugars, and Explants?

In Vitro Micro Tuber Formation in Potato (Solanum tuberosum L.): is there any Relation between Methyl Jasmonate, Sugars, and Explants?

 
 		 International Journal of Biotech Trends and Technology (IJBTT)
 
© 2018 by IJBTT Journal
Volume - 8 Issue - 1                          
Year of Publication : 2018
Authors : Gülsün E. Vural, Tugce Ozsan, Volkan Gozen, Ahmet N. Onus
DOI :  10.14445/22490183/IJBTT-V8I1P601

Citation

Gülsün E. Vural, Tugce Ozsan, Volkan Gozen, Ahmet N. Onus "In Vitro Micro Tuber Formation in Potato (Solanum tuberosum L.): is there any Relation between Methyl Jasmonate, Sugars, and Explants?", International Journal of Biotech Trends and Technology (IJBTT), V8(1): 1-8 January - March 2018, Published by Seventh Sense Research Group.

Abstract

Studies on potato (Solanum tuberosum L.) plants have been carried out to plant tissue culture laboratories because of the disease and virulent free seed tubers in field conditions. Since generative production in commercial potato production is not economically massive, production is made more economically with tubers. Jasmonic acid (JA) and its related compounds are newly recognized plant growth regulators and they are known to have effects on many physiological processes. In this study, it was aimed to determine the effects of methyl jasmonate and sucrose on micro tuber formation in potato micropropagation. To serve the purpose the relationships between twelve media combinations (including control medium), varying concentrations of sucrose (3, 6 and 9 %) either alone or in combination with 1.0 ppm or 2.0 ppm methyl jasmonate were investigated. Three different plant parts such as node, shoot eye and primordial tissue wereused as explant sources. Nitsch&Nitsch was used as the basic nutrient medium and plant growth regulators that used in media were constant at 2.5 mg/L BAP + 0.5 mg/L NAA except for control medium. Obtained micro tubers, plantlets and callus formations were recorded and analyzed. Results revealed that, when methyl jasmonate used at 1.0 ppm concentration in media, it had a positive effect on micro tuber formation and can be recommended to use commercially in mass production.

References

[1] (2016) The FAO website. [Online]. Available: http://www.fao.org/
[2] G. Hussey, & N. J. Stacey, “In vitro propagation of potato (Solanum tuberosum L.),” Ann. Bot., vol. 48, pp. 787-796, 1981.
[3] S. Khuri and J. Moorby, “Nodal segments or microtubers as explants for in vitromicrotuber production of potato,” Plant Cell, Tissue and Organ Culture, vol. 45, pp. 215-222, 1996.
[4] B. H. McCown and P. J. Joyce, “Automated propagation of microtubers of potato,” In Scale-Up and Automation in Plant Propagation, I.K. Vasil, Ed., pp. 95-109. Academic Press. San Diego, CA. 1991.
[5] D. J. Donnelly, W. K. Coleman & S. E. Coleman, “Potato microtuber production and performance: A review,” Amer. J. Potato Res., vol. 80, pp. 103-115, 2003.
[6] M. Kane, “Micropropagation of Potato by Node Culture and Microtuber Production,” In: Plant Tissue Culture, Development, and Biotechnology, Robert N. Trigiano, Dennis J. Gray Ed., pp. 207-212. CRC Press.USA, 2011.
[7] J. E. A. Seabrook, S. Coleman, and D. Levy, “Effects of photoperiod on in vitrotuberization of potato (Solanum tuberosum L.),” Plant Cell Tiss. Organ Cult.vol. 34, pp. 43-51, 1993.
[8] J. Gopal, J. L. Minocha& H. S. Dhaliwal, “Microtuberization in potato (Solanum tuberosum L.)," Plant Cell Rep., vol. 17, pp. 794-798, 1998.
[9] W. M. Roca, N. O. Espinoza, M. R. Roca & J. E. Bryan, “A tissue culture method for the rapid propagation of potatoes,” Amer. Potato J.,vol 55, pp. 691-701, 1978.
[10] R. B. Copeland, “Micropropagation of potatoes,” Agric. Northern Ireland, vol. 57, pp. 250-253, 1982.
[11] N. O. Espinoza, R. Estrada, D. Silva-Rodriguez, P. Tovar, R. Lizarraga& J. H. Dodds, “The potato: A model crop plant for tissue culture,” Outlook on Agriculture, vol. 15, pp. 21-26, 1986.
[12] B. S. Ahloowalia, “Production and performance of potato mini-tubers,” Euphytica, vol. 75, pp. 163-172, 1994.
[13] G. Hussey & N. J. Stacey, “Factors affecting the formation of in vitro tubers of potato (Solanum tuberosum L.),” Annals of Botany, vol. 53, pp. 565-578, 1984.
[14] R. Estrada, P. Tovar & J. H. Dodds, “Induction of in vitro tubers in a broad range of potato genotypes,” Plant, Cell, Tissue and Organ Culture, vol. 7, pp. 3-10, 1986.
[15] P. O. Ovono, C. Kevers, J. Dommes, “Effects of reducing sugar concentration on in vitro tuber formation and sprouting in yam (Dioscoreacayenensis – D. rotundata complex),” Plant Cell Tiss Organ Cult., vol. 99, pp. 55–59, 2009. DOI 10.1007/s11240-009-9575-1.
[16] C. Wasternack, “Jasmonates: an update on biosynthesis, signal transduction and action in plant stress response, growth and development,” Annals of Botany, vol. 100, pp. 681–697, 2007.
[17] Y. Koda, Y. Kikuta, H. Tazaki, Y. Tsujino, S. Sakamura, T. Yoshihara, “Potato tuber-inducing activities of jasmonic acid and related compounds,” Phytochernistry, vol. 30, pp. 1435-1438, 1991.
[18] Jeffrey C. Suttle& Linda L. Huckle& Edward C. Lula, “The Effects of Dormancy Status on the Endogenous Contents and Biological Activities of Jasmonic Acid, N-(jasmonoyl)-Isoleucine, and Tuberonic Acid in Potato Tubers,” Am. J. Pot Res. Vol. 88, pp. 283–293, 2011. DOI 10.1007/s12230-011-9192-5.
[19] ?. Bakt?r, “Jasmonateler (YaseminAsitleri),” Hormonlar, BitkiGeli?imDüzenleyicileri, ÖzellikleriveTar?mdaKullan?mlar?, sf. 90-92, HasadYay?nc?l?k, ?stanbul, Türkiye, 2015.
[20] L. I. Martin-Closas, S. Sol and A. M. Pelacho, “Potential application of jasmonic acid for Solanum tuberosummicropropagation,” XXV. International Horticultural Congress, Part 10: Application of Biotechnology and Molecular Biology and Breeding-In vitro Culture, Brussels, Belgium (Eds. L. H. W. van der Plas and G. J. de Klerk). ISHS ActaHorticulturae vol. 520, pp. 127-134, 2000.
[21] Y. Koda and Y. Kikuta, “Effects of jasmonates on in vitrotuberization in several potato cultivars that differ greatly in maturity,” Plant Prod.Sci., vol 4, pp. 66–70, 2001.
[22] A. M. Kumlay, “The Effect of Jasmonic Acid on the Micropropagation of Potato (Solanum tuberosum L.)Under Long Days Conditions,” YYÜ TAR B?L DERG (YYU J AGR SCI), vol. 26, pp. 79-88, 2016.
[23] J. P. Nitsch and C. Nitsch, “Haploid plants from pollen grains,” Science, vol. 163, pp. 85- 87, 1969.
[24] Y. Koda, “Possible involvement of jasmonates in various morphogenic events,” Physiol Plant, vol. 100, pp. 639–646, 1997.
[25] D. Sarkar, S. K. Pandey, S. Sharma, “Cytokinins antagonize the jasmonate action on the regulation of potato (Solanum tuberosum) tuber formation in vitro,” Plant Cell Tissue Organ Cult., vol. 87, pp. 285–295, 2006. DOI: 10.1007/s11240-006-9166-3.
[26] P. O. Ovono, C. Kevers, J. Dommes, “Tuber formation and growth of Dioscoreacayenensis-D. rotundata complex: interactions between exogenous and endogenous jasmonic acid and polyamines,” Plant Growth Regul, vol. 60, pp. 247–253, 2010. DOI 10.1007/s10725-009-9441-5.
[27] K. Pruski, T. Astatkie, J. Nowak, “Jasmonate on in vitrotuberization and tuber bulking in two potato cultivars (Solanum tuberosum L.) under different media photoperiod conditions,” In vitro Cell Dev. Biol. Plant., vol. 38, pp. 203–209, 2002.
[28] A. M. Pelacho, A. M. Mingo-Castel, “Jasmonic acid induces tuberisation of potato stolons cultured in vitro,” Plant Physiol. Vol. 97, pp. 1253-1255 1991.
[29] E. A. Janet, L. Seabrook, K. Douglass, & D. A. Arnold, “Effect of Leaves on Microtubers Produced from Potato Single-Node Cuttings In Vitro,” Amer J of Potato Res., vol. 81, pp. 1-5, 2004.
[30] T. Murashige, F. Skoog, “A revised medium for rapid growth and bioassays with tobacco tissue culture,” Physiol. Plant.vol. 15, pp. 473-497, 1962.
[31] N. Garner & J. Blake, “The induction and development of potato microtubersin vitro on media free of growth regulating substances,” Ann Bot, vol. 63, pp. 663-674, 1989.
[32] D. Sarkar and P. S. Nalk, “Effect of Inorganic nitrogen nutrition on cytokinin-induced potato microtuber production in vitro,” Potato Res, vol. 41, pp. 211-217, 1998.
[33] G. F. Stallknecht and S. Farusworth, “The effect of nitrogen on the coumarin-induced tuberization of potato axlllary shoots cultured in vitro,” Am Potato J, vol. 56, pp. 523-530, 1979.
[34] A. Zarrabeitia, X. Lejarcegui, J. Veramendi, A. M. Mingo Castel, “Influence of nitrogen supply on micropropagation and subsequent microtuberization of four potato cultivars,” Am. Pot. J.,vol. 74, pp. 369-378, 1997.
[35] P. Wang and C. Hu, “In vitro mass tuberization and virus-free seed potato production in Taiwan,” Am Potato J., vol. 59, pp. 33-37, 1982.
[36] S. Khuri and J. Moorby, “Investigations into the role of sucrose in potato cvEstimamicrotuber production in vitro,” Ann Bot, vol. 75, pp. 295-303, 1995.
[37] A. J. Abbott & A. R. Belcher, “Potato tuber formation in vitro” In: LA Withers, PG Alderson (Eds.). Plant Tissue Culture and its Agricultural Applications, pp. 113-122, Butterworths, London, 1986.
[38] F. Corbineau, R. M. Rudnicki& D. Come, “The effects of methyl jasmonate on sunflower seeds germination and seedling development,” Plant Growth Regul., vol. 7:, pp. 157-169, 1988.
[39] S. Q. Huang, J. H. Bin & Z. P. Li, “Effects of methyl jasmonate and ABA on the growth of root and hypocotyls of peanut seedling,” J. Plant Physiol. mol. Biol., vol. 28, pp. 351-356, 2002.
[40] M. Ravnikar, B. Vilhar and N. Gogala, “Stimulatory effects of jasmonic acid on potato node and protoplast culture,” J Plant Growth Reg., vol. 11, pp. 29-33, 1992.
[41] V. Repka, I. Fischerová, K. Šilhárová, “Methyl jasmonate is a potent elicitor of multiple defense responses in grapevine leaves and cell-suspension cultures,” Biol. Plant, vol. 48, pp. 273- 283, 2004.
[42] Z. J. Zhang, W. J. Zhou, H. Z Li, G. Q. Zhang, K. Subrahmaniyan and J. Q. Yu, “Effect of jasmonic acid on in vitro explant growth and microtuberization in potato,” BiologiaPlantarum, vol. 50, pp. 453 - 456, 2006.
[43] S. D. Jackson & L. Willmitzer, “Jasmonic acid spraying does not induce tuberisation in short-day-requiring potato species kept in non-inducing,” Planta, vol. 194, pp. 155-159, 1994.
[44] S. Biondi, S. Fornale, K. M. Oksman-Caldentey, M. Eeva, S. Agostani& N. Bagni, “Jasmonates induce over-accumulation of methylputrescine and conjugated polyanfines in Hyoscyamusmuticus L. root cultures,” Plant Cell Rep, vol. 19, pp. 691-697, 2000.
[45] K. Pruski, T. Astatkie, P. Duplessis, T. Lewis, J. Nowak and R. C. Struik, “Use of Jasmonate for Conditioning of Potato Plantlets and Microtubers in Greenhouse Production of Minitubers,” Amer J of Potato Res, vol. 80, pp. 183-193, 2003.
[46] P. Tovar, R. Estrada, L. Schilde-Rentschler and J. H. Dodds, “Induction and use of in vitro potato tubers,” International Potato Centre, CIP Circular, vol. 13, pp. 1-5, 1985.

Keywords
Solanum tuberosumL., micro tuber, methyl jasmonate, sucrose, in vitro.