Development of Modified Medium for Enhanced Production of Lipase by Streptomyces Halstedii Strain ST 70 Obtained From Bhitarkanika Mangroves

Development of Modified Medium for Enhanced Production of Lipase by Streptomyces Halstedii Strain ST 70 Obtained From Bhitarkanika Mangroves

 
 		 International Journal of Biotech Trends and Technology (IJBTT)
 
© 2014 by IJBTT Journal
Volume - 4 Issue - 4                          
Year of Publication : 2014
Authors : Srilekha Mishra , Nibha Gupta

Citation

Srilekha Mishra , Nibha Gupta. "Development of Modified Medium for Enhanced Production of Lipase by Streptomyces Halstedii Strain ST 70 Obtained From Bhitarkanika Mangroves", International Journal of Biotech Trends and Technology (IJBTT), V4(4):1-7 Oct - Dec 2014, Published by Seventh Sense Research Group.

Abstract

The lipase production by Streptomyces halstedii strain ST 70, a mangrove isolate was enhanced through the modifications of cultural conditions and nutritional amendments. The strain produced 26.1 U/mL enzyme in presence of starch, glycine and ammonium chloride that was enhanced upto 56.1 U/ml with 50% ammonium sulphate precipitation. Results showed that tween 80 is required as inducer to enhance the lipase production. The enzyme was found to be stable at various temperature and pH where as saline condition did not favour higher enzyme activity. The present study reveals importance towards the industrial level exploitation of this strain due to its origin and only few Streptomyces strains are reported for lipase production.

References

[1] M Abramic , I.Lescic, T Korika, L Vitale, W Saenger, J Pigac, Purification and properties of extracellular lipase from Streptomyces rimosus. Enzyme Microb. Technol. 25:522-529, (1999).
[2] R Bancerz, G Ginalska, J Fiedurek, A Gromada, Cultivation conditions and properties of extracellular crude lipase from the psychrotrophic fungus Penicillium chrysogenum 9. J of Ind Microbiol Biotechnol , 32:253-260, (2005).
[3] S Benjamin, A Pandey, Coconut cake: a potent substrate for the production of lipase by Candida rugosa in solid-state fermentation. Acta Biotechnol, 17: 241-251, (1997).
[4] H Chander, and H Klostermeyer, Production of lipase by Byssochlamys fulva. J.food prot., 46:707-709, (1983).
[5] N Christova, B Tuleva, D Galabova, Dependence of yeast permabilization with TritonX-100 on the cell age. Biotechnol Tech 10: 77-78, (1996).
[6] E Dalmau, J.L Montesinos, M Lotti, C Cassas, Effect of different carbon sources on lipase production by Candida rugosa. Enzyme Microb.Technol.26:657-663,(2000).
[7] R Gandolfi, F Marinelli, A Lazzarini, Molinari, F, Cell bound and extracellular carboxylesterases from Streptomyces: hydrolytic and synthetic activities. J. Appl. Microbiol.89:870-875, (2000).
[8] M.L Gaspar, M. Cunningham, R.Pollero, M Cabello, Occurrence and Properties of an Extracellular lipase in Mortierella vinacea. Mycologia 91:108-113 (1999).
[9] Z Genovefa, E Wenzig , A. Mersmann, A Improvement of lipase production by addition of catalase during fermentation. Appl Microbiol Biotechnol 40: 650-652, (1994).
[10] E.H Ghanem , A.S Hashim , M.K. Saleh An alkalophilic thermostable lipase produced by a new isolate of Bacillus alcalophilus. World J Microbiol Biotechnol. 16 , (2000).
[11] M.L.F. Giuseppin , Effects of dissolved oxygen concentration on lipase production by Rhizopus delemar .Appl Microbiol Biotechnol 20: 161-165, (1984).
[12] K.P Large, N Mirajili, M.O.L.M Peacock, V Zormpaidis, M.Walsh, M.E Cavanagh , P.F.Leadlay , A.P Ison, Lipase activity in Streptomyces- the role of lipase interfacial binding. Enzyme Microb. Technol. 25:569 575, (1999).
[13] K.P Large, N Mirjalili, M Osborne, L.M Peacock, V Zompaidis, M Walsh, M.E Cavanagh, P.F Leadlay, A.P. Ison Lipase activity in Streptomyces.EnzymeMicrob.Technol.25:569-575(1999).
[14] I Lescic, Vukelic B, M.Majeric Elenkov, W Saenger, M.Abramic, Substrate specificity and effects of water-miscible solvents on the activity and stability of extracellular lipase from Streptomyces rimosus. Enzyme Microb.Technol. 29: 548-553, (2001).
[15] I Lescic, l M Zeh, R. Muller, B.Vukelic , M. Abramic, J Pigac, G Allmaier, B Kojicprodic, Structural characterization of extra cellular lipase from Streptomyces rimosus: assignment of disulphide bridge pattern by mass spectrometry. Biol Chem. 385: 1147-1156, (2004).
[16] D.W Lee, Y.S Koh, K J Kim, B.C Kim, H. J Choi, D.S Kim, M.T.Suhartono, Y.R Pyun, Isolation and characterization of thermophillic lipase from Bacillus thermoleovoransID1. FEMS microbiol. Lett 179: 393-400, (1999).
[17] C.Y.Li, C.Y Cheng, T.L Chen, Production of Acinetobacter radioresistens lipase using Tween-80 as the carbon source. Enzyme Microb.Technol. 29:258-263, (2001).
[18] C.Y.Li, C.Y. Cheng, T.L Chen, Fed batch production of lipase by acinetobacter radioresistens using Tween-80 as the carbon source. Biochem. Eng. J.19: 25-31, (2004).
[19] V. M.G Lima, N.Krieger, M.I.M Sarquis, D.A Mitchell, L.P.Ramos, J.D. Fontana, Effect of Nitrogen and Carbon sources on lipase production by Penicillium aurantiogriseum. Food Technol. Biotechnol. 41: 105-110, (2003).
[20] ]K Long, H.M Ghazali, A Ari, K Ampon, C Bucke, Mycelium bound lipase from a locally isolated strain of Aspergillus flavus link: pattern and factors involved in its production. J Chem Tech Biotechnol ,67:157-163,(1996).
[21] M.Maia , M Morais, M Antonio, E Melo, L Jose, Production of extra cellular lipase by the phytopathogenic fungus Fusarium solani FS1. Revista de microbiologia 30:304-309,(1999).
[22] ]F Molinari, D.Romano, R Gandolfi, R.M Kroppenstedt., F Marinelli , Newly isolated Streptomyces spp. as enantioselective biocatalysts: hydrolysis of 1,2-O-isopropylidene glycerol recemic esters. J. Appl. Microbiol. 99: 960-967(2005).
[23] C Perez, K Juarez, E Gracia-castells, G Soberon , L Servin-Gonzalez ,.Cloning, characterization, and expression in Streptomyces lividans 66 of an extracellular lipase encoding gene from Streptomyces sp. M11. Society of General Microbiology 145:2365-2374(1999).
[24] ]S.E Petrovic, M Skrinjar, A Becaravic, I.F Vujucic, L Banka.Effect of various carbon sources on microbial lipases biosynthesis. Biotechnol Lett 12: 299-304, (1990).
[25] P Rapp, S Backhaus, Formation of extracellular lipases by filamentous fungi, yeasts and bacteria. Enzyme Microb Technol 14:938-943, (1992).
[26] P Rathi, S Bradoo, R.K Saxena, R.Gupta, A hyper thermostable alkaline lipase from Pseudomonas sp. with the property of thermal activation. Biotechnol. Lett. 22:495-498,(2000).v [27] N Ray, L Ray, B.N Srimani , P Chattopadhyay, Isolation and identification of alkaline thermostable lipase producing microorganism, cultural conditions, nutritional requirements and some properties of crude enzyme. Ind. J Expt Biol. 37: 818-824,(1999.).
[28] J Sharma, R Kumar, A Singh, Production of alkaline lipase by Aspergillus fumigatus using solid-state fermentation of products obtained from grain processing. Ind J Microbiol. 44: 141-143,(2004).
[29] P Sommer, C Bormann, F Gotz ,Genetic and biochemical characterization of a new extracellular lipase from Streptomyces cinnamomeus. Appl.Environ.Microbiol. 63: 3553-3560. ,(1997).
[30 ] H,Sztajer, I Maliszewska, J Wieczorek Production of exogenous lipases by bacteria, fungi and actinomycetes. Enzyme Microb.Technol.10:492-497,(1988).
[31] C Tesch, K Nikoleit, V Gnau, F Gotz,C Bormann, Biochemical and Molecular characterization of the extracellular esterase from Streptomyces diastatochromogenes. J Bacteriol.178:1858-1865,(1996).
[32] M Uyeda, M Hirotsu, M Itonaga, S Urata, K Suzuki, M Shibata, Purification and Properties of Lipase activator produced by Streptomyces sp. strain no. BR-1381. Agric.Biol.Chem.47:2739-46,(1983).
[33] D Vujaklija, M.Abramic, L Ivana, M Tereza, P Jasenka, Streptomyces rimosus GDS (L) lipase: Production, Heterologus Over expression and Structure Stability Relationship. Food TechnolBiotechnol.41:89-93,(2003).
[34] ]D Wei, L Zhang , Q Song, Studies on a novel carbon source and co solvent for lipase production by Candida rugosa. Ind J Microbiol. Biotechnol. 31:133-136,(2004).
[35] Y Wei , L.J.Schottel , U, Derewenda, , L Swenson, S Patkar, Z.S Derewenda ,A Novel variant of the catalytic triad in the Streptomyces scabies esterase. Nature Structural Biology 2: 218-223,(1995).
[36] H Xiang, N Takaya, T Hoshino, Novel cholesterol Esterase Secreted by Streptomyces Persists during Aqueous Long-Term Storage. J. Biosci. Bioeng. 101: 19-25,(2006).
[37] X Yang, B Wang, F Cui, T Tan, Production of lipase by repeated batch fermentation with immobilized Rhizopus arrhizius. Process Biochem. 40: 2095-2103,(2005).

Keywords
Lipase, Streptomyces, mangrove, nutrient, carbon source, nitrogen source