Isolation of bacteria producing cellulase from tilapia fish gut and media optimization for celluase production using Plackett Burman design

 
 
International Journal of Biotech Trends and Technology (IJBTT)
 
© 2017 by IJBTT Journal
Volume - 7 Issue - 2                         
Year of Publication : 2017
Authors : Sandeep Chovatiya; Snehal Ingle, Dipika Patel, Bhoomi Thakkar
  10.14445/22490183/IJBTT-V21P603

Sandeep Chovatiya; Snehal Ingle, Dipika Patel, Bhoomi Thakkar "Isolation of bacteria producing cellulase from tilapia fish gut and media optimization for celluase production using Plackett Burman design", International Journal of Biotech Trends and Technology (IJBTT), V7(2): 13-18 Apr to Jun 2017, Published by Seventh Sense Research Group.

Abstract

Cellulase production from bacteria can be an advantage as the enzyme production rate is normally higher due to the higher bacterial growth rate as compared to fungi. Screening of bacteria, optimisation of fermentation conditions and selection of substrates are important for the successful production of cellulase. This study is conducted to produce cellulase from tilapia fish gut bacteria, using Plackett Burman design and carboxymethyl cellulose (CMC) as substrate. The highest cellulase producing ability of isolate from this study was found to be 0.323 IU/ml with 24 hours of incubation. With the help of Plackett-Burman design, maximum cellulolytic activities was observed in high levels of CMC, Urea, Peptone, Calcium chloride, magnesium sulphate and Trace elements and Low levels of Ammonium sulphate, and Di Potassium Hydrogen phosphate, at 37 °C and pH 7.0 which is found to be optimized levels of components.

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Keywords
Celluase, fish gut, tilapia, Plackett Burman design.