Studies on Development of High Yielding Ethanol and Temperature Resistance Strain of Saccharomyces cerevisiae

 
 
International Journal of Biotech Trends and Technology (IJBTT)
 
© 2015 by IJBTT Journal
Volume-13                         
Year of Publication : 2015
Authors : Biswanath Biswas, A.K. Banik and Asit Baran Biswas

Biswanath Biswas, A.K. Banik and Asit Baran Biswas "Studies on Development of High Yielding Ethanol and Temperature Resistance Strain of Saccharomyces cerevisiae", International Journal of Biotech Trends and Technology (IJBTT),V13: 20-24 November-December 2015. Published by Seventh Sense Research Group.

Abstract

Saccharomyces cerevisiae AB, an ethanol producing strain (3.0 %) was treated with 10%, 15% and 20% ethanol for 30, 60,120 and 180 minute. Only 5.5% colonies isolated by 30 minute. Incubation in 10% ethanol gave higher alcohol production (5.4%) than the parent strain. This strain when incubated in 15% ethanol for 60 minute, then only 4.5% alcohol resistant strains gave higher alcohol production (6.3%). The parent strain on farther exposure to 20% ethanol, there was decreases in alcohol production. Saccharomyces cerevisiae AB 810 an ethanol production strain (6.3%) was incubated at 35 0C, 40 0C and 45 0c temperature for 6, 12, 18 and 24 hours. Only 18% colonies isolated by 24 hour incubation at 350C temperature which gave highest ethanol production (6.5%). On further exposure at 45 0C temperature, all the resistant strains were killed, so there was no alcohol production.

References

[1] Laluce, C., Bertolini, M.C., Ernasdes, J.R., Martini, A., and Martini A.V., Ann. Microbial Enzimol (Milan) 37, 151-159 (1987).
[2] Emandes, J.R., Matulionis, M. , Cruz, S.H., Bertolini, M.C., and Laluce,C., Bitechnol. Lett. 12, 463-468 (1990).
[3] Cecilia Laluce et al, Biotechnology and Bioengg., 37,528-536 (1991).
[4] Camelia Bonciu, Cristiana Tabacaru and Gabriela Bahrim, 6, 29-34 (2010).
[5] Mobini- Dehkordi, M., Nahvi, I., Ghaedi, K., and Tavassoli, M.,Research in Pharmaceutical sciences2, 85-91 (2007).
[6] Del Rosario E.J., Lee K.J. and Rogers P.L., Biotechnol Bioengg., 21, 1477 (1979).
[7] Holzberg I., Finn R. and Steinkrauss K., Biotechnol Bioengg, 9, 413, (1967).
[8] Cysewski G.R. and Wilke C. R., Bioetechnol Bioengg. 18, 1297, (1976).
[9] Alexandre, H., Costello, P.J., Remize, F, Guzzo, J. And Guilloux-Benatitr, M., Inst. J. Food Microbiol 93, 141, (2004).
[10] Chaney, D., Rodriguez, S., Fugelsang, K. And Thorutan, J. Appl. Microbiol., 100, 689 (2006).
[11] Perez, M., Luyten, K., Mlichel, R. and Blonalin, B., FEMS yeast Research, 5,351 (2005).
[12] D’Amore, T., et al., Enzyme. Microb. Technol. 11:411-416 (1989).
[13] Association of official Agricultural Chemists, official and Tentative Methods of Analysis, 6th ed., Washington D.C., Association of Official Agricultural Chemists, (1950).

Keywords
Saccharomyces cerevisiae, alcohol resistant and temperature resistant, ethanol production.