IJBTT

Comparison of Regeneration Efficiency of Three Different Genotypes of Basmati Rice under in Vitro Condition

	International Journal of Biotech Trends and Technology (IJBTT)
	© 2015 by IJBTT Journal
	Volume - 5 Issue - 2
	Year of Publication : 2015
	Authors : Ritu Mahajan, Shilpi Sharma

Citation

Ritu Mahajan, Shilpi Sharma "Comparison of Regeneration Efficiency of Three Different Genotypes of Basmati Rice under in Vitro Condition", International Journal of Biotech Trends and Technology (IJBTT), V5(2):16-24 Apr - Jun 2015, Published by Seventh Sense Research Group.

Abstract

Seeds of three basmati rice genotypes namely Pusa Sugandha, Pusa1 and Pusa 1121 were assessed for callus induction and plant regeneration on different concentrations and combinations of plant growth regulators in MS medium. A two stage sterilization treatment was found to be the most effective leading to maximum number of uncontaminated explants in three rice genotypes. The number of shoots was observed to be more in MS media supplemented with BAP and the shoot length was more in MS media supplemented with Kn. An optimum concentration of 1.0mg/l of 2,4-D was found to be effective for callus induction but Pusa Sugandha had more potential towards callus formation than Pusa 1 and Pusa 1121. However, highest percentage of shoot regeneration from callus was observed in Pusa Sugandha (90.0%) followed by Pusa 1 (80.0%) and Pusa 1121 (50.0%) in MS media supplemented with 0.5 mg/l of 2,4-D, 1.0 mg/l of Kn and 0.5 mg/l of BAP.

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Keywords
Oryza sativa, basmati, in vitro, callus, growth regulators, genotypes